Bioactivity Metabolite from the Endophytics Penicillium citrinum and P. polonicum of Portulaca oleracea Against Ralstonia solanacearum
Author:
  • Article
  • | |
  • Metrics
  • |
  • Reference [34]
  • |
  • Related [20]
  • | | |
  • Comments
    Abstract:

    To investigate the diversity of endophytics in medicinal plant Portulaca oleracea and explore potential biological resources to prevent and control crop diseases, the endophytes from its roots, stems and leaves were isolated by medium cultivation method, their bioactivities were evaluated against Ralstonia solanacearum, and they were identified by colonial morphology and ITS sequence. The results showed that the crude extracts of two endophytics Penicillium citrinum and P. polonicum displayed strong activities against the phytopathogen R. solanacearum. One of active metabolite (citrinin) was identified based on UPLC-QTQF-MS. Moreover, the activity of citrinin had more strong antibacterial property than that of streptomycin, which was a common agent in agriculture to prevent bacterial wilt. So, these would provide scientific basis for biological control of bacterial wilt.

    Reference
    [1] GAO H L. The research progress of Ralstonia solanacearum[J]. Legal Syst Soc, 2009(12):354. doi:10.19387/j.cnki.1009-0592.2009.12.244. 高欢乐. 论青枯菌病害的研究进展[J]. 法制与社会, 2009(12):354. doi:10.19387/j.cnki.1009-0592.2009.12.244.
    [2] LI T W. Advances in biological control of Ralstonia solanacearum[J]. Plant Doct, 2009, 22(5):4-6. doi:10.3969/j.issn.1007-1067.2009.05.001. 李土文. 青枯病的生物防治研究进展[J]. 植物医生, 2009, 22(5):4-6. doi:10.3969/j.issn.1007-1067.2009.05.001.
    [3] WANG Z W, JI Y L, CHEN Y G. Studies and biological significances of plant endophytes[J]. Microbiol China, 2015, 42(2):349-363. doi:10.13344/j.microbiol.China.130815. 王志伟, 纪燕玲, 陈永敢. 植物内生菌研究及其科学意义[J]. 微生物学通报, 2015, 42(2):349-363. doi:10.13344/j.microbiol.China. 130815.
    [4] CHEN W P. Experimental study on the antibacterial effect of Portulaca oleracea extract liquid[J]. Lishizhen Med Mat Med Res, 2007, 18(9):2205-2206. doi:10.3969/j.issn.1008-0805.2007.09.078. 陈万平. 马齿苋提取液体外抑菌作用的实验研究[J]. 时珍国医国药, 2007, 18(9):2205-2206. doi:10.3969/j.issn.1008-0805.2007.09.078.
    [5] FAN Y S. Study on the hypoglycemic activity of polysaccharides isolated from Portulace oleracea L.[D]. Yangling:NW Agric For University, 2008:1-66. 范玉生. 马齿苋多糖降血糖作用研究[D]. 杨凌:西北农林科技大学, 2008:1-66.
    [6] HE G P. Pharmacological action and clinical application of Portulaca oleracea L.[J]. Chin J Drug Abuse Prev Treat, 2009, 15(4):243-245. doi:10.15900/j.cnki.zylf1995.2009.04.002. 何国萍. 马齿苋的药理作用与临床应用[J]. 中国药物滥用防治杂志, 2009, 15(4):243-245. doi:10.15900/j.cnki.zylf1995.2009.04.002.
    [7] LIU H, LI Y P, ZHOU C L, et al. Study on extraction and bioactivity of Portulaca oleracea L. flavonoids[J]. Lishizhen Med Mat Med Res, 2010, 21(11):2866-2867. doi:10.3969/j.issn.1008-0805.2010.11.069. 刘华, 李玉萍, 周春丽, 等. 马齿苋黄酮提取及生物活性研究[J]. 时珍国医国药, 2010, 21(11):2866-2867. doi:10.3969/j.issn.1008-0805.2010.11.069.
    [8] QIN X Z, WANG D, ZHANG H Y, et al. Study on the anti-inflam-mation, analgesia effects and the toleration oxygen deficit of the porslane[J]. Chin J Hosp Pharm, 2010, 30(22):1909-1911. 秦孝智, 王丹, 张红英, 等. 马齿苋不同提取物的抗炎镇痛耐缺氧作用[J]. 中国医院药学杂志, 2010, 30(22):1909-1911.
    [9] LI W K, HU Z B. Endophytes and natural medicines[J]. Chin Nat Med, 2005, 3(4):193-199. 黎万奎, 胡之璧. 内生菌与天然药物[J]. 中国天然药物, 2005, 3(4):193-199.
    [10] JIA L, CHEN S Y, ZHAI Y G, et al. Recent advances in studies on endophytes and their associated bioactive products[J]. Chin Trad Herb Drugs, 2007, 38(11):1750-1754. doi:10.3321/j.issn:0253-2670.2007.11.053. 贾栗, 陈疏影, 翟永功, 等. 近年国内外植物内生菌产生物活性物质的研究进展[J]. 中草药, 2007, 38(11):1750-1754. doi:10.3321/j. issn:0253-2670.2007.11.053.
    [11] STIERLE A, STROBEL G, STIERLE D. Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew[J]. Science, 1993, 260(5105):214-216. doi:10.1126/science.8097061.
    [12] REN A Z, GAO Y B. Endophytic fungi, a kind of resource micro-organism with broad application prospect[J]. Microbiol China, 2001, 28(6):90-93. doi:10.3969/j.issn.0253-2654.2001.06.022. 任安芝, 高玉葆. 植物内生真菌——一类应用前景广阔的资源微生物[J]. 微生物学通报, 2001, 28(6):90-93. doi:10.3969/j.issn.0253-2654.2001.06.022.
    [13] LIU J H, YU B Y. Isolation of endophytic fungi from Camptotheca acuminata and the screening method of antitumor secondary meta-bolite produced by the fungi[J]. J Plant Resour Environ, 2004, 13(4):6-10. doi:10.3969/j.issn.1674-7895.2004.04.002. 刘吉华, 余伯阳. 喜树内生真菌的分离及其抗肿瘤活性代谢产物的筛选方法[J]. 植物资源与环境学报, 2004, 13(4):6-10. doi:10.3969/j. issn.1674-7895.2004.04.002.
    [14] ZHANG J C, WANG Z M, ZHANG H Y, et al. The summarize about recent research process on fern endophyte[J]. Chin Agric Sci Bull, 2010, 26(20):70-72. 张君诚, 王铮敏, 张杭颖, 等. 蕨类植物内生菌研究进展[J]. 中国农学通报, 2010, 26(20):70-72.
    [15] GONG J. Isolation and anti-microbial activity of endophyte in Portu-laca oleracea L.[J]. Food Ind, 2014, 35(7):144-147. 巩健. 马齿苋内生菌分离及其抑菌效果研究[J]. 食品工业, 2014, 35(7):144-147.
    [16] ZHAO F F, HAN B J, LI P P, et al. Extracts from tendrils and leaves of Piper nigrum Linn.:Components analysis and activity to three types of agricultural pathogenic fungi[J]. Chin J Trop Crops, 2019, 40(1):115-122. doi:10.3969/j.issn.1000-2561.2019.01.017. 赵方方, 韩丙军, 李萍萍, 等. 胡椒蔓和叶粗提物成分分析及对3种农业病原真菌活性研究[J]. 热带作物学报, 2019, 40(1):115-122. doi:10.3969/j.issn.1000-2561.2019.01.017.
    [17] XIAO Y R, WANG Y S, LI N Z, et al. Isolation and identification of a blinin-producing endophytic fungus from Conyza blinii H. Lév[J/OL]. Genom Appl Biol, 2019:1-8.[2019-04-04] http://kns.cnki.net/kcms/detail/45.1369.Q.20190403.1421.004.html. 肖义蓉, 王寅生, 李南臻, 等. 金龙胆草产苦蒿素内生真菌的筛选鉴定[J/OL]. 基因组学与应用生物学, 2019:1-8.[2019-04-04] http://kns.cnki.net/kcms/detail/45.1369.Q.20190403.1421.004.html.
    [18] ZHANG Y. Isolation of endophytic fungi from Portulaca oteracea and study on their infulence on hosts growth[D]. Hohhot:Inner Mongolia Agricultural University, 2015:1-39. 张瑜. 马齿苋内生真菌的分离、鉴定及其对宿主生长的影响[D]. 呼和浩特:内蒙古农业大学, 2015:1-39.
    [19] WU Y, SHANG S H, YANG Q, et al. Isolation and identification of endophytes from Portulaca oteracea[J]. J Shanxi Agric Univ (Nat Sci), 2014, 34(2):117-120. 武燕, 尚世辉, 杨倩, 等. 马齿苋内生细菌的分离鉴定[J]. 山西农业大学学报(自然科学版), 2014, 34(2):117-120.
    [20] NAMBOODIRI M M T, PAKSHIRAJAN K. Sustainable and green approach of chitosan production from Penicillium citrinum biomass using industrial wastewater as a cheap substrate[J]. J Environ Manage, 2019, 240:431-440. doi:10.1016/j.jenvman.2019.03.085.
    [21] OUEPHANIT C, BOONVITTHYA N, THEERACHAT M, et al. Effi-cient expression and secretion of endo-1,4-β-xylanase from Penicillium citrinum in non-conventional yeast Yarrowia lipolytica directed by the native and the preproLIP2 signal peptides[J]. Protein Expr Purif, 2019, 160:1-6. doi:10.1016/j.pep.2019.03.012.
    [22] NIKITINA Z K, GORDONOVA I K. Exogenous regulation of produ-ctivity of Penicillium citrinum-keratinase producer[J]. Probl Biol Med Pharm Chem, 2016, 19(9):8-12.
    [23] CHEN X Y, WANG B, PAN L. Heterologous expression and charac-terization of Penicillium citrinum nuclease P1 in Aspergillus niger and its application in the production of nucleotides[J]. Protein Expr Purif, 2019, 156:36-43. doi:10.1016/j.pep.2018.12.004.
    [24] MUTHUKRISHNAN S. Optimization and production of industrial important cellulase enzyme from Penicillium citrinum in Western Ghats of Sathuragiri hills soil sample isolate[J]. Univ J Microbiol Res, 2017, 5(1):7-16. doi:10.13189/ujmr.2017.050102.
    [25] QADER M M, KUMAR N S, JAYASINGHE L, et al. Production of antitumor antibiotic GKK1032B by Penicillium citrinum, an endo-phytic fungus isolated from Garcinia mangostana fruits[J]. Med Aromat Plants, 2016, 5(1):1000225. doi:10.4172/2167-0412.1000225.
    [26] MANI P G, AUDIPUDI A V. Growth stimulation and induction of systemic resistance in chilli against anthracnose disease by Penicillium citrinum AVGE5[J]. Int J Adv Res, 2016, 4(5):419-426.
    [27] ALVES A M, de MOURA R B, CARVALHO A K F, et al. Penicillium citrinum whole-cells catalyst for the treatment of lipid-rich wastewater[J]. Biomass Bioenergy, 2019, 120:433-438. doi:10.1016/j.biombioe. 2018.12.004.
    [28] WEN Y Z, LV Y B, HAO J, et al. Two new compounds of Penicillium polonicum, an endophytic fungus from Camptotheca acuminata Decne[J/OL]. Nat Prod Res, 2019,[2019-02-13] doi:10.1080/14786419.2019.1569003.
    [29] FATIMA N, SRIPISUT T, YOUN U J, et al. Bioactive constituents from an endophytic fungus, Penicillium polonicum NFW9, associated with Taxus fauna[J]. Med Chem, 2017, 13(7):689-697. doi:10.2174/1573406413666170216145121.
    [30] ELSUNNI M A, YANG Z D. Secondary metabolites of the endophytic fungi Penicillium polonicum and their monoamine oxidase inhibitory activity[J]. Chem Nat Compd, 2018, 54(5):1018-1019. doi:10.1007/s10600-018-2540-7.
    [31] NEETHU S, MIDHUN S J, SUNIL M A, et al. Efficient visible light induced synthesis of silver nanoparticles by Penicillium polonicum ARA 10 isolated from Chetomorpha antennina and its antibacterial efficacy against Salmonella enterica serovar Typhimurium[J]. J Photo-chem Photobiol B, 2018, 180:175-185. doi:10.1016/j.jphotobiol. 2018.02.005.
    [32] NEETHU S, MIDHUN S J, RADHAKRISHNAN E K, et al. Green synthesized silver nanoparticles by marine endophytic fungus Penici-llium polonicum and its antibacterial efficacy against biofilm forming, multidrug-resistant Acinetobacter baumanii[J]. Microb Pathog, 2018, 116:263-272. doi:10.1016/j.micpath.2018.01.033.
    [33] HAYES A W, BIRKHEAD H E, HALL E A. Antiprotozoal activity in citrinin[J]. Bull Environ Contam Toxicol, 1976, 15(4):429-436. doi:10.1007/BF01685067.
    [34] WANG Y. Citrinin[J]. Sci China, 1951, 2(4):433-454. 汪猷. 橘霉素[J]. 中国科学, 1951, 2(4):433-454.
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

张倩,黎平,何子康,颜健.马齿苋内生菌橘青霉和波兰青霉中抗青枯菌的活性物质[J].热带亚热带植物学报,2019,27(6):731~738

Copy
Share
Article Metrics
  • Abstract:737
  • PDF: 557
  • HTML: 407
  • Cited by: 0
History
  • Received:March 14,2019
  • Revised:April 18,2019
  • Online: November 29,2019
Article QR Code