Gene Cloning and Expression Analysis of BRI1 in Eucalyptus grandis

Abstract:

In order to understand the function of BRI1 gene in Eucalyptus grandis, EgrBRI1 gene was cloned, and the bioinformatics and subcellular localization of EgrBRI1 were analysis. Meanwhile, the expression of EgrBRI1 gene were analyzed after treated with hormones and stresses by qRT-RCR. The results showed that the length of EgrBRI1 gene was 3 893 bp, encoding 1 197 amino acids. EgrBRI1 protein was relatively stable with complex spatial structure, which contains three motifs. EgrBRI1 protein was mainly located in cell membrane. When treated with methyl jasmonate and brassinolide, the expression of EgrBRI1 gene in leaves showed an up-regulated trend with the time, while there was no significant change by treated with salicylic acid. Under salt and cold stresses, the expression of EgrBRI1 gene was down-regulated at first and then increased with the time. These indicated that EgrBRI1 gene could respond quickly to applied hormone and play an important role in stress resistance of E. grandis, which might be achieved by responding to BR signal.

Key words:Eucalyptus grandis;Brassinosteroid;EgrBRI1 gene;Gene expression