Abstract:In order to screen excellent germplasm of Hylocereus undatus, the fruit genetic diversity analysis in phenotypic traits, agronomic traits and quality traits of 22 germplasms were studied. The results showed that the phenotypic, agronomic and quality traits of H. undatus fruit had abundant genetic diversity and high variability. The diversity indexes (H') of fruit phenotypic and quality traits were 0-1.04 and 0.40-2.01, respectively. The coefficient of variation (CV) of agronomic traits and quality traits were 0.01-0.62 and 0.06-0.38, respectively. Cluster analysis showed that 22 germplasms of H. undatus could be devided into 5 groups at genetic distance of 15, indicating that different germplasms were far related. These would lay a foundation for exploring the breeding potential of H. undatus, screening excellent genetic resources and improving germplasm.