Home > Archive>Volume 27, Issue 4, 2019 >408-414. DOI:10.11926/jtsb.4002
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Functional Analyses of WUBE1 Gene Isolated from Citrus reticulata ‘Wuzishatangju’
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    Abstract:

    In order to understand the role of UBE1 gene in self-incompatibility (SI) response of Citrus reticulata ‘Wuzishatangju’, a WUBE1 gene from the self-incompatible cultivar 'Wuzishatangju' was transferred into tobacco (Nicotiana tabacum) mediated by Agrobacterium tumefaciens. The results showed that the WUBE1 gene was integrated into genome and expressed in transgenic tobacco. Some pollen tubes of self-pollinated transgenic tobacco with WUBE1 became twisted and could not enter the embryo sac. The number of pollen tube entering into ovules in self-pollinated WUBE1 tobacco was fewer than that in wild type (WT) and cross-pollination (transgenic tobacco×WT). However, there was no significant difference in pollen viability, germination rate and seed number per pod between transgenic tobacco and WT. Therefore, it was suggested that only the WUBE1 could not regulate SI response of ‘Wuzishatangju’, and it was possible to form a complex in Ub/26S pathway involved in the SI reaction.

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李鹏,苗红霞,胡桂兵,秦永华.无籽沙糖桔WUBE1基因的功能验证[J].热带亚热带植物学报,2019,27(4):408~414

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History
  • Received:October 08,2018
  • Revised:November 15,2018
  • Online: August 08,2019
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