Transcriptome Analysis of Mesembryanthemum crystallinum under Salt Stress

Abstract:

In order to understand the salt related genomics in leaves of Mesembryanthemum crystallinum, the expression of transcriptome genes was studied under 400 mmol L^-1 NaCl stress by using Illumina Hiseq TM2500 high-throughput sequencing technology. The results showed that there were 13.01 Gb Clean Data from the leaves treated with 400 mmol L^-1 NaCl and untreated, the Q30 base was more than 90.08%. Among them, there were 123 differential expression genes (DEGs), including 73 up-regulated genes and 50 down-regulated genes, in which 96 genes were annotated. The eight salt-resistance DEGs of were screened out based on GO, COG and KEGG annotation. The plant hormone metabolism-related genes, including abscisic acid 8'-hydroxylase gene, indole-3-acetamide synthase gene, jasmonate ZIM structure domain protein gene were down-regulated, auxin responsive protein and cytokinin synthase genes were up-regulated. The sugar metabolism-related raffinose synthase genes was up-regulated, and plasma membrane H⁺-ATPase and dehydrated protein genes were up and down-regulated, respectively. These would provide a foundation for studing on salt tolerance genomics and molecular biology of M. crystallinum.

Key words:Mesembryanthemum crystallinum;Salt stress;Leaf;Transcriptome;DEGs