Abstract:In order to understand the endophytes deversity in Jasminum sambac (L.) Ait, the endophytes component was determined based on high throughput sequencing of 16S rDNA. The results showed that the endophytes in J. sambac was very rich, belonging to 38 phyla, 78 classes, 150 orders, 257 families and 434 genera. The Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes and Chloroflexi had high abundance. The abundance of Proteobacteria was the highest from June to October, accounting for 70.3%-85.7%. The Shannon index and Simpson index of endophytes were the biggest (3.14) and the smallest (0.13) in July, respectively, showing the highest bacterial diversity, which suggested that high temperature was beneficial to increase endophytes diversity. According to clustering and principal component analysis, the endophytes community structure in plants had high similarity in July and August, September and October, while that in June were obviously different from those in other months. Heatmap and redundancy analysis indicated that endophytes diversity were singificantly influenced by soil nutrient ingredients, and the contents of organic matter, total phosphorus and nitrogen were major factors. Therefore, these would provide a foundation for developing benefical endophytes from J. sambac.