Transcriptome Analysis of Transcription Factors of Rhododendron pulchurum ‘Baifeng4’ under Different Water Conditions

Abstract:

In order to reveal the expression pattern of transcriptome factors (TFs) of Rhododendron pulchurum under drought stress, the expression profiles of transcription factors in ‘Baifeng 4’ under different water conditions were analysed by using the high-throughput sequencing technology. The results showed that there were 34-161 differential expression TFs among different water treatments. The transcriptional regulation in Rhododendron response to drought was synergistic effects of the expressions of ERF, bHLH and MYB genes. The special expression of NAC and up expression of WRKY, bZIP, PLATZ were found under drought stress, and the special expression of GATA in rehydration. The trend of gene expression was consistent with sequencing results by RT-qPCR, showing the validity of sequencing results. Therefore, these would be lay a theoretical foundation for understanding the molecular mechanism of drought resistance and molecular breeding of Rhododendron.

Key words:Rhododendron;Transcription factor;Transcriptome;Drought stress