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Studies on Genetic Diversity of Juglans Cultivar Germplasms in Sichuan Based on RAD-SNPs Analysis
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    Abstract:

    In order to understand the genetic diversity of walnut (Juglans) in Sichuan, the genetic diversity and genetic structure of 42 walnut germplasms were analyzed by RAD-SNPs method. The results showed that a total of 70G clean data and 160 309 high consistent SNPs were obtained, and the average Q30 was 96.3%. Forty-two walnut genotypes could be divided into two main groups, such as AJR (J. regia group, n=33) and AJS (J. sigillata group, n=9). The high genetic differentiation (FST=0.285) between two groups had correlation with their geographic distribution. Meanwhile, the groups of three varieties of ‘Weibo No.1’, ‘Bailong No.1’ and ‘Shimianjuhe’ were adjusted. There were 9 and 14 varieties in AJS and AJR groups with pure blood, respectively, while the rests in AJS and AJR groups were mixed blood. There were high genetic diversity among 42 walnut germplasms with nucleotide diversity[Pi(π)] of 0.029 and expected heterozygosity (He) of 0.286, and the J. sigillata group has higher genetic diversity than J. regia group. So, these would provide basis for the preservation and cross breeding of walnut germplasm resources in Sichuan.

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闫思宇,朱鹏,龚伟,王景燕,吴开志,吴春艳,李海平,龚毅红,段琼.基于RAD-SNPs分析的四川核桃良种资源的遗传多样性研究[J].热带亚热带植物学报,2019,27(1):19~28

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  • Received:March 15,2018
  • Revised:May 09,2018
  • Online: January 19,2019
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