Cloning and Expression of LcUGE in Liriodendron chinense

Abstract:

In order to understand the function of UGE in Liriodendron chinense, two genes, named LcUGE1 and LcUGE2, were obtained by RACE and EPIC-PCR. The result showed that the full-length cDNA of LcUGE1 and LcUGE2 were 1 531 and 1 378 bp with 1 050 and 1 056 bp open reading frame (ORF), and encoding 349 and 351 amino acids, respectively. Their genome DNA length were 11 920 and 6 544 bp, respectively. Both of LcUGE1 and LcUGE2 genes contained 9 exons and 8 introns. Though the exon length and intron splicing site sequence were almost similar, thier intron fragment length were different. The nucleic acid sequences of LcUGE1 and LcUGE2 were highly conserved up to 82% similarity. The expression of LcUGE1 and LcUGE2 were the highest in stamens and calyx, respectively. Therefore, the LcUGE genes could be involved in reproductive development of L. chinense.

Key words:Liriodendron chinense;LcUGE;Gene clone;Gene expression