Home > Archive>Volume 26, Issue 6, 2018 >627-632. DOI:10.11926/jtsb.3878
PDF HTML XML Export Cite reminder
Changes in Composition of Anthocyanins in Brunfelsia acuminata Flowers
Author:
  • Article
    • | |
  • Metrics
    • |
  • Reference [17]
    • |
  • Related [20]
    • |
  • Cited by
    • | |
  • Comments
    Abstract:

    In order to understand the mechanism of flower colour change of Brunfelsia acuminate, the changes in composition of anthocyanins in petals were studied by using high performance liquid chromatography (HPLC) system. The results showed that the optimized HPLC system was following:the flow rate was 0.8 mL min-1, 7.5% formic acid acetonitrile as mobile phase A and 7.5% formic acid as mobile phase B; the elution program was 0 min with 8% A; 15 min with 18% A; 25 min 23% A; 45 min with 40% A and 50 min with 8% A. There were three kinds of anthocyanins, such as malvidin-3-O-glucoside chloride, petunidin-3-glucoside and delphinidin-3-glucoside in petals of B. acuminata by using the optimized system. Among them, the content of malvidin-3-O-glucoside chloride was the highest, followed by petunidin-3-glucoside and the content of delphinidin 3-glucoside was the lowest. Moreover, the contents of three kinds of anthocyanin decreased with the petal color from dark to light. Therefore, it was indicated that the coloration of B. acuminata is related to these three anthocyanins, and the malvidin-3-O-glucoside chloride plays a dominant role.

    Reference
    [1] Vaknin H, Bar-akiva A, Ovadia R, et al. Active anthocyanin degradation in Brunfelsia calycina (yesterday-today-tomorrow) flowers[J]. Planta, 2005, 222(1):19-26. doi:10.1007/s00425-005-1509-5.
    [2] CHEN X Q, GUO Z X, LIN L, et al. POD isozyme system optimi-zation of Brunfelsia acuminate (Solanaceae) in different development stages[J]. Chin J Trop Crops, 2014, 35(8):1510-1516. doi:10.3969/j.issn.1000-2561.2014.08.011. 陈小琴, 郭志雄, 林琳, 等. 鸳鸯茉莉不同发育时期POD同工酶体系优化[J]. 热带作物学报, 2014, 35(8):1510-1516. doi:10.3969/j. issn.1000-2561.2014.08.011.
    [3] ZHAI S H, ZOU X J, DING L, et al. Effect of different pruning treatments on florescence of Brunfelsia latifolia[J]. J Kunming Univ, 2014, 36(3):61-63. doi:10.14091/j.cnki.kmxyxb.2014.03.024. 翟书华, 邹晓菊, 丁丽, 等. 不同修剪措施对鸳鸯茉莉花期的影响[J]. 昆明学院学报, 2014, 36(3):61-63. doi:10.14091/j.cnki.kmxyxb. 2014.03.024.
    [4] de ROSAS I, PONCE M T, MALOVINI E, et al. Loss of anthocyanins and modification of the anthocyanin profiles in grape berries of Malbec and Bonarda grown under high temperature conditions[J]. Plant Sci, 2017, 258:137-145. doi:10.1016/j.plantsci.2017.01.015.
    [5] CAO Y T. Cloning the full length cDNA of anthocyanins biosynthasis key genes and CHS gene promoter from Brunfelsia acuminate flowers[D]. Fuzhou:Fujian Agriculture and Forestry University, 2012:1-82. 曹玉婷. 鸳鸯茉莉花瓣色素合成关键酶基因cDNA及CHS基因启动子的克隆[D]. 福州:福建农林大学, 2012:1-82.
    [6] CAO Y T, QIU D L. Cloning and sequence analysis of chalcone isomerase gene (CHI) from Brunfelsia acuminata flowers[J]. J Trop Subtrop Bot, 2012, 20(5):475-481. doi:10.3969/j.issn.1005-3395.2012.05.008. 曹玉婷, 邱栋梁. 鸳鸯茉莉查尔酮异构酶基因(CHI) cDNA的克隆与生物信息学分析[J]. 热带亚热带植物学报, 2012, 20(5):475-481. doi:10.3969/j.issn.1005-3395.2012.05.008.
    [7] LI M, CAO Y T, YE S R, et al. Isolation of CHS gene from Brunfelsia acuminata flowers and its regulation in anthocyanin biosysthesis[J]. Molecules, 2017, 22(1):44-53. doi:10.3390/molecules22010044.
    [8] LI M, YE S R, QIU D L. Optimization of two-dimensional electro-phoresis technology for Brunfelsia acuminate petals[J]. Subtrop Agric Res, 2015, 11(4):262-266. doi:10.13321/j.cnki.subtrop.agric.res.2015. 04.009. 李敏, 叶丝蕊, 邱栋梁. 鸳鸯茉莉花瓣蛋白质双向电泳技术的优化[J]. 亚热带农业研究, 2015, 11(4):262-266. doi:10.13321/j.cnki. subtrop.agric.res.2015.04.009.
    [9] YUAN Y, LIAN F Q. A preliminary study on the callus of Brunfelsia latifolia induced by different explants[J]. N Hort, 2010(1):168-171. 袁媛, 连芳青. 鸳鸯茉莉不同外植体诱导愈伤组织研究初探[J]. 北方园艺, 2010(1):168-171.
    [10] BAR-AKIVA A, OVADIA R, ROGACHEV I, et al. Metabolic net-working in Brunfelsia calycina petals after flower opening[J]. J Exp Bot, 2010, 61(5):1393-1403. doi:10.1093/jxb/erq008.
    [11] SIMÕES C, BIZARRI C H B, DA SILVA CORDEIRO L, et al. Anthocyanin production in callus cultures of Cleome rosea:Modu-lation by culture conditions and characterization of pigments by means of HPLC-DAD/ESIMS[J]. Plant Physiol Biochem, 2009, 47(10):895-903. doi:10.1016/j.plaphy.2009.06.005.
    [12] WEISS D, HALEVY A H. Stamens and gibberellin in the regulation of corolla pigmentation and growth in Petunia hybrida[J]. Planta, 1989, 179(1):89-96. doi:10.1007/BF00395775.
    [13] da FONSECA MACHADO A P, PEREIRA A L D, BARBERO G F, et al. Recovery of anthocyanins from residues of Rubus fruticosus, Vaccinium myrtillus and Eugenia brasiliensis by ultrasound assisted extraction, pressurized liquid extraction and their combination[J]. Food Chem, 2017, 231:1-10. doi:10.1016/j.foodchem.2017.03. 060.
    [14] WANG F, YANG S H, LIU X Y, et al. The diversity of petal colors and its relation to anthocyanins in Rose germplasms[J]. Acta Hort Sin, 2017, 44(6):1125-1134. doi:10.16420/j.issn.0513-353x.2016-0789. 王峰, 杨树华, 刘新艳, 等. 月季种质资源花色多样性及其与花青苷的关系[J]. 园艺学报, 2017, 44(6):1125-1134. doi:10.16420/j.issn. 0513-353x.2016-0789.
    [15] WANG L, LIU Y L, LOU Q, et al. Method development for the identification of anthocyanins in Muscari latifolium flower[J]. Acta Bot Boreali-Occid Sin, 2017, 37(5):1031-1037. doi:10.7606/j.issn.1000-4025.2017.05.1031. 王琳, 刘雅莉, 娄倩, 等. 葡萄风信子花青素苷分析方法建立及成分鉴定[J]. 西北植物学报, 2017, 37(5):1031-1037. doi:10.7606/j.issn. 1000-4025.2017.05.1031.
    [16] ZHANG J, WANG L S, GAO J M, et al. Identification of anthocyanins involving in petal coloration in Chaenomeles speciosa cultivars[J]. Acta Hort Sin, 2011, 38(3):527-534. 张洁, 王亮生, 高锦明, 等. 贴梗海棠花青苷组成及其与花色的关系[J]. 园艺学报, 2011, 38(3):527-534.
    [17] SUN W, Li C H, WANG L S, et al. Anthocyanins present in flowers of Senecio cruentus with different colors[J]. Acta Hort Sin, 2009, 36(12):1775-1782. doi:10.16420/j.issn.0513-353x.2009.12.011. 孙卫, 李崇晖, 王亮生, 等. 花青苷成分对瓜叶菊花色的影响[J]. 园艺学报, 2009, 36(12):1775-1782. doi:10.16420/j.issn.0513-353x. 2009.12.011.
    Cited by
Get Citation

李敏,罗宇婷,卢小草,孙月婷,邱栋梁.鸳鸯茉莉开花过程中花青素组成的变化[J].热带亚热带植物学报,2018,26(6):627~632

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:January 12,2018
  • Revised:March 29,2018
  • Online: November 21,2018
Article QR Code
You are the first4648341 Visitors
Governing Body：中国科学院
Organizer：中国科学院华南植物园 广东省植物学会
Address：Xingke Road 723,Tianhe District,Guangzhou,China
Phone：+86 (0)20-3725 2514
Journal of Tropical and Subtropical Botany ® 2025 All Rights Reserved