Abstract:To understand the regulatory function of cold resistance transcription factor ICE1 in Canarium album, one ICE1 gene, named CaICE1, was cloned from ‘Fulan-1’ by using RT-PCR, and its bioinformatic characteristic, expression pattern and predicted targeting miRNAs were studied. The results showed that the length of open reading frame of CaICE1 was 1 650 bp, encoding 549 amino acids (GenBank accession No. MG459422). The CaICE1 was an unstable and hydrophilic protein located at nucleus, with transmembrane structure, phosphorylation sites and conserved HLH domains, and which had close relationship with ICE1 of Citrus trifoliata. Although the codon bias level was low, AGA, AGG, TGG and CCA would be optimal codons of CaICE1. The expression of CaICE1 was high in flower, seed and leaf, and which was significantly up-regulated under -3℃ compared with room temperature. Additionally, CaICE1 was predicted to be targeted by several stress responsive miRNAs, such as miR825, miR477, miR5658, miR1436 and miR394. Therefore, it was suggested that CaICE1 might play important regulation roles in low temperature stress of C. album, and its expression might be regulated by some stress responsive miRNAs.
