Construction of Rapid Propagation System in vitro of Iris hexagona

Abstract:

In order to construct the rapid propagation system in vitro of Iris hexagona, the effects of medium and plant growth regulators on induction, proliferation and rooting of adventitious buds were studied by using young rhizome as explant. The results showed that the rhizome was sterilized for 13 min with 0.1% HgCl₂. The optimum medium for induction was MS+1.5 mg L^-1 6-BA+0.5 mg L^-1 NAA+30 g L^-1 sucrose+7.5 g L^-1 agar, and that for adventitious bud proliferation was MS+0.5 mg L^-1 6-BA+0.2 mg L^-1 NAA+0.3 mg L^-1 KT+30 g L^-1 sucrose+7.5 g L^-1 agar. The rooting rate of plantlets could reach up to 100% cultured on MS+1.5 mg L^-1 IBA+ 30 g L^-1 sucrose+7.5 g L^-1 agar. The survival of plantlets could reach up to 100% transplanted on humic soil or perlite+peat soil+vermiculite (1∶2∶1) as substrate.

Key words:Iris hexagona;Rapid propagation in vitro;Medium;Plant growth regulator