Nematicidal Activity of Chemical Compositions from Aristolochia tuberosa Fruits against Root-knot Nematode

Abstract:

In order to understand the bioactivity constituents from Aristolochia tuberosa O. F. Liang et S. M. Huang, four compounds were isolated from methanol extract of A. tuberosa fruits by using silica gel column chromatography, Sephadex LH-20 gel column chromatography, precipitation and recrystallization methods. On the basis of spectral data, their structures were identified as aristololactam W (1), 8-epidiosbulbin e acetate (2), diosbulbin B (3) and β-sitosterol (4). Furthermore, nematicidal activity of four compounds against the second stage juvenile (J2) of Meloidogyne javanica were analyzed. The compounds 1-3 exhibited obviously toxic effects on J2 of M. javanica in vitro, especially for aristololactam W with the LC₅₀ values of 119.94 μg mL^-1 after 96 h. So, Aristolochia species have the potential value of developing new plant source to control root nematodes.