Studies on Embryogenic Callus Induction and Plant Regeneration of Anthurium andreanum

Abstract:

In order to construct regeneration system from embryogenic callus of Anthurium andreanum, the tender leaves and petioles of 3 cultivars were used as explants, the effects of medium, plant growth regulator combination and illumination condition on embryogenic callus induction and plant regeneration were studied. The results showed that the optimum medium for embryogenic callus induction was modified MS₃+1.5 mg L^-1 2,4-D+0.5 mg L^-1 KT+4% sucrose+2% glucose+0.25% Phytagel. The embryogenic callus induction rate had obvious difference among cultivars and explants, showing in the order of ‘Pink Champion’ > ‘Robino’ > ‘Champion’, and leaves > petioles. The embryogenic callus induction rate of ‘Pink Champion’ leaves could reach up to 57.9%. The optimum medium for embryoid differentiation was 1/2 modified MS_a+2% sucrose+0.25% Phytagel. The embryoid differentiation rate derived from leaves of ‘Pink Champion’ could reach up to 31.6%, and had not significant differences cultured under light and dark. The survival could reach up to 100% after transplanted plantlets.