Home > Archive>Volume 26, Issue 3, 2018 >249-254. DOI:10.11926/jtsb.3824
PDF HTML XML Export Cite reminder
Effect of Cryopreservation on Structure, Physiology and Biochemical Characteristics of Dipterocarpus turbinatus Seeds
Author:
  • Article
    • | |
  • Metrics
    • |
  • Reference [22]
    • |
  • Related
    • | | |
  • Comments
    Abstract:

    In order to preserve effectively and stably the seeds of Dipterocarpus turbinatus Gaertn. F., the influence of low temperature storage on seed vigor, structure and physiological and biochemical indexes was studied. The results showed that the water content of seeds with 45% was safe for storage. The vitrification and slow freezing had more damage to seed structure, and the seed vigor decreased greatly. So, the best method for cryopreservation of seeds was the rapid freezing. The liquid nitrogen frozen reduced the activities of POD, CAT and dehydrogenase, and increased the activity of α-amylase, but had no significant effects on the content of MDA. Therefore, ultralow temperature preservation is scientific and feasible method for the seeds of D. turbinatus.

    Reference
    [1] WANG Y, QIU Q, YANG D J, et al. Technique for Dipterocarpus trubinatus' introduced cultivation and growth process of its plantation[J]. For Invent Plan, 2010, 35(1):135-138. doi:10.3969/j.issn.1671-3168.2010.01.035. 王艳, 邱琼, 杨德军, 等. 羯布罗香引种栽培技术及其人工林生长过程研究[J]. 林业调查规划, 2010, 35(1):135-138. doi:10.3969/j.issn.1671-3168.2010.01.035.
    [2] ZHOU L, HUANG Z Y, HUANG J P. The "God mollis":Diptero-carpus trubinatus[J]. Garden, 2013, 1(2):66-67. doi:10.3969/j.issn. 1000-0283.2013.02.017. 周亮, 黄紫云, 黄建平. 傣家的"神油树":羯布罗香[J]. 园林, 2013, 1(2):66-67. doi:10.3969/j.issn.1000-0283.2013.02.017.
    [3] YANG D J, QIU Q, WEN J, et al. The cultivation technology in hilly area and sapling growth of Dipterocarpus trubinatus[J]. China For Sci Technol, 2008, 22(3):79-82. doi:10.3969/j.issn.1000-8101.2008.03.025. 杨德军, 邱琼, 文进, 等. 羯布罗香山地栽培技术及幼林生长规律[J]. 林业科技开发, 2008, 22(3):79-82. doi:10.3969/j.issn.1000-8101.2008.03.025.
    [4] ROBERTS E H. Problems of long-term storage of seed and pollen for genetic resources conservation[M]//FRANKEL O H, HAWKES J G. Crop Genetic Resources for Today and Tomorrow. London:Cambridge University Press, 1975:269-294.
    [5] LIU Z F. Seed Science:A Laboratory Manual[M]. Beijing:Chemical Industry Press, 2011:62-104. 刘子凡. 种子学实验指南[M]. 北京:化学工业出版社, 2011:62-104.
    [6] ZHANG H R, The Principle and Method of Biochemistry Experiment[M]. Yinchuan:Ningxia People's Publishing Press, 1999:59-63. 张慧茹. 生物化学试验原理和方法[M]. 银川:宁夏人民出版社, 1999:59-63
    [7] FU J R, SONG S Q. Biology of Recalcitrant Seeds[M]. Beijing:China Science Culture Press, 2004:60-61. 傅家瑞, 宋松泉. 顽拗性种子生物学[M]. 北京:中国科学文化出版社, 2004:60-61.
    [8] TIAN X M, LI H L, HE Y, et al. Research progress on recalcitrant seeds storage of tropical crops[J]. Chin J Trop Agric, 2014, 34(8):52-58. doi:10.3969/j.issn.1009-2196.2014.08.011. 田新民, 李洪立, 何云, 等. 热带作物顽拗型种子保存研究进展[J]. 热带农业科学, 2014, 34(8):52-58. doi:10.3969/j.issn.1009-2196. 2014.08.011.
    [9] YANG H G. Characteristics of germination of Piper nigrum and Coffea canephora seeds stored at an ambient temperature[J]. Chin J Trop Agric, 2006, 26(6):7-9,12. doi:10.3969/j.issn.1009-2196.2006.06.003. 杨华庚. 胡椒和咖啡2种中间性种子的萌发特性研究[J]. 热带农业科学, 2006, 26(6):7-9,12. doi:10.3969/j.issn.1009-2196.2006.06.003.
    [10] YE F, XIA S, MEI X G. Studies on factors in cryopreservation of Taxus callus[J]. J Wuhan Bot Res, 2001, 19(4):327-331. doi:10.3969/j.issn. 2095-0837.2001.04.008. 叶芳, 夏胜, 梅兴国. 红豆杉愈伤组织超低温保存有关因素的研究[J]. 武汉植物学研究, 2001, 19(4):327-331. doi:10.3969/j.issn.2095-0837.2001.04.008.
    [11] CHENG Z Y. Physiological and biochemical and structure charac-teristics of chrysanthemum shoot tips during cryopreservation[D]. Nanjing:Nanjing Agricultural University, 2006:3-4. 程志英. 菊花(Dendranthema morifolium)茎尖超低温处理过程中的相关生理生化与结构变化[D]. 南京:南京农业大学, 2006:3-4.
    [12] SHI R, WANG F, WANG J. Advances in vitrification cryopreservation technology[J]. N Hort, 2011(24):231-235. 石茹, 王芳, 王舰. 玻璃化法超低温保存技术及其研究进展[J]. 北方园艺, 2011(24):231-235.
    [13] ZENG L, HE M J, ZHENG X L, et al. Study on cryopreservation of Alpinia officinarum Hance seeds[J]. Chin Agric Sci Bull, 2014, 30(28):164-148. 曾琳, 何明军, 郑希龙, 等. 高良姜种子超低温保存研究[J]. 中国农学通报, 2014, 30(28):164-148.
    [14] LIU J M, XU Z Q, XU H H, et al. Study on cryopreservation of seeds of Aquilaria sinensis (Lour.) Gilg.[J]. J Guangzhou Univ Trad Chin Med, 2007, 24(5):414-415. doi:10.3969/j.issn.1007-3213.2007.05.017. 刘军民, 徐梓勤, 徐鸿华, 等. 白木香种子的超低温保存研究[J]. 广州中医药大学学报, 2007, 24(5):414-415. doi:10.3969/j.issn. 1007-3213.2007.05.017
    [15] ZENG L, HE M J, WEI J H, et al. Cryopreservation study on seeds and embryos in Dalbergia odorifera T. Chen[J]. China J Chin Mat Med, 2014, 39(12):2263-2266. doi:10.4268/cjcmm20141222. 曾琳, 何明军, 魏建和, 等. 降香黄檀种子和离体胚超低温保存研究[J]. 中国中药杂志, 2014, 39(12):2263-2266. doi:10.4268/cjcmm 20141222.
    [16] ZENG L, WU Y, HE M J, et al. Physiological and biochemical characteristics of Alpinia oxyphylla seeds after cryopreservation[J/OL]. Guihaia, 2017:1-12[2017-06-13]. http://kns.cnki.net/kcms/detail/45.1134.Q.20170613.1758.002.html. doi:10.11931/guihaia.gxzw201704031. 曾琳, 吴怡, 何明军, 等. 超低温冷冻对益智种子生理生化特性的影响[J]. 广西植物, 2017:1-12[2017-06-13]. http://kns.cnki.net/kcms/detail/45.1134.Q.20170613.1758.002.html. doi:10.11931/guihaia. gxzw201704031
    [17] SONG H, CAO B, WANG L. Physiological and biochemical charac-teristics of Iris ensata seeds after cryopreservation[J]. J NE For Univ, 2016, 44(4):44-47. 宋红, 曹柏, 王玲. 超低温保存对玉蝉花种子生理生化特性的影响[J]. 东北林业大学学报, 2016, 44(4):44-47.
    [18] GAO R Q, ZHANG C Q. Seed Biology[M]. Beijing:China Science and Technology Press, 2002:201-202. 高荣岐, 张春庆. 种子生物学[M]. 北京:中国科学技术出版社, 2009:201-202.
    [19] QIAN H G. Study on seed germination characteristics and seed vigor changes of three elm species[D]. Yangling:Northwest Agricultural and Forestry University, 2009:7-9. 钱红格. 三种榆属树种种子萌发特性及活力变化的研究[D]. 杨凌:西北农林科技大学, 2009:7-9.
    [20] CHEN H, HAN X Y. Influence on physiological and biochemical characteristics of crisp plum seeds after cryopreservation[J]. Tianjin Agric Sci, 2013, 19(5):1-4. 陈红, 韩晓莹. 超低温保存对贵州酥李种子生理生化特性的影响[J]. 天津农业科学, 2013, 19(5):1-4.
    [21] ZHAO Y J, WANG T. Analysis of the relationship between α-amylase and germinating rate of rice seeds during the process of seed germi-nation[J]. Chin Bull Bot, 2001, 18(2):226-230. 赵玉锦, 王台. 水稻种子萌发过程中α-淀粉酶与萌发速率关系的分析[J]. 植物学通报, 2001, 18(2):226-230.
    [22] TANG Z J, SONG M. Physiological and biochemical analysis of artificially aged Chinese cabbage[Brassica campestris L. ssp. Pekin-ensis (Lour.) Olsson][J]. Acta Hort Sin, 1999, 26(5):319-322. 唐祖君, 宋明. 大白菜种子人工老化及劣变的生理生化分析[J]. 园艺学报, 1999, 26(5):319-322.
    Related
    Cited by
    Comments
    Comments
    分享到微博
    Submit
Get Citation

曾琳,顾雅坤,吴怡,郑希龙.超低温冷冻对羯布罗香种子结构和生理生化特性的影响[J].热带亚热带植物学报,2018,26(3):249~254

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:September 18,2017
  • Revised:November 15,2017
  • Online: May 29,2018
Article QR Code
You are the first4648123 Visitors
Governing Body：中国科学院
Organizer：中国科学院华南植物园 广东省植物学会
Address：Xingke Road 723,Tianhe District,Guangzhou,China
Phone：+86 (0)20-3725 2514
Journal of Tropical and Subtropical Botany ® 2025 All Rights Reserved