Abstract:In order to search the chemical constituents of Artabotrys hexapetalus, twelve alkaloid compounds were isolated from the stems of A. hexapetalus. On the basis of spectal data, they were identified as stepholidine (1), tetrahydrocolumbamine (2), tetrahydrojatrorrhizine (3), pronuciferine (4), glaziovina (5), sinoacutine (6), pallidine (7), stepharine (8), artacinatine (9), lanuginolide (10), isoscoulerine (11) and N-methylasimilobine (12). The alkaloids 1-7 were isolated from the genus Artabotrys for the first time. Compound 10 showed potent antibacterial effects on Staphylococcus albus and Bacillus cereus, with MIC of 20 and 10 μg mL-1, respectively.