Abstract:In order to selective breeding and seedling production of Dendrobium officinale, the pollination success rate, fruit-set rate and seed germination rate of 46 germplasms were studied by using artificial pollination, including autogamy, geitonogamy, crossing among different plants within the same germplasm and crossing among different germplasms. The results showed that the pollination was successful by crossing between different germplasms, and the fruit setting rate were high. While there were only 31.3% germplasms successful by self-pollination, and the fruit setting rate had markedly difference. The pollination success rate and fruit setting rate of geitonogamy and crossing among different plants within the same germplasm were both intervenient. And the same changes were observed in seed germination rate and speed as well as the fruit setting rate among 4 types of pollinations. These indicated that self-incompatibility existed in D. officinale germplasms, so that the pollination success rate, fruit setting rate and seed germination rate could increase markedly by crossing between different germplasms.