Abstract:Gynura bicolor is rich in anthocyanidin. In order to identify the transcription factors involved in anthocynidin biosynthesis pathway in G. bicolor, the transcriptome of this plant was sequenced by Illumina HiSeq 2500 platform. By searching of 3 databases, including Pfam, SwissProt and Nr, 138 MBW-related unigenes were obtained, which containing 42 MYBs, 67 bHLHs, 15 bHLH-MYBs and 14 WD40s. Among these MBWs, 11 MYBs, 33 bHLHs, 6 bHLH-MYBs and 3 WD40s involved in anthocynidin biosynthesis pathway were annotated in other plant species, including Vitis vinifera, Dahlia pinnata, Gerbera, Catharanthus roseus, Prunus persica etc. The obtaining of the 138 unigenes related to MBWs from Gynura bicolor provides a good basis for further research in gene cloning and the regulation of anthocyanin synthesis.