Abstract:In order to screen fungi with promoting effect on Artemisia annua growth, the endophytic fungi were isolated from A. annua brunches by tissue isolation method and molecular identified. The promoting effect of Trichoderma atroviride was studied. The results showed that 23 endophytic fungi were isolated belonging to 16 speices, in which the genus Colletotrichum was dominant. The fermentation broth of T. atroviride had significantly negative effect on seed germination and seedling growth of A. annua The mycelium of T. atroviride liquid fermentation had significant promoting effect on seedling growth of A. annua during April to May, but did not after July. So, it was suggested that T. atroviride could be used as fertilizer for seedlings of A. annua, and it combining with chemical fertilizer could also be applied to adult plants.