Isolation and Molecular Identification of Endophytic Fungi from Artemisia annua and Promoting Effect of Trichoderma atroviride on Its Growth

doi:10.11926/jtsb.3768

Home > Archive>Volume 26, Issue 1, 2018 >56-64. DOI:10.11926/jtsb.3768

Isolation and Molecular Identification of Endophytic Fungi from Artemisia annua and Promoting Effect of Trichoderma atroviride on Its Growth
DOI:
                        10.11926/jtsb.3768
                    
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                        WU Xiao-liWU Xiao-li
1. Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;2. Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica, Chongqing 400065, China;3. Chongqing Subcenter of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Science, Chongqing 400065, China
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CUI Guang-linCUI Guang-lin
1. Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;2. Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica, Chongqing 400065, China;3. Chongqing Subcenter of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Science, Chongqing 400065, China
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LIU FeiLIU Fei
Central Laboratory, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China
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LI Long-yunLI Long-yun
1. Institute of Material Medical Planting, Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China;2. Chongqing Engineering Research Center for Fine Variety Breeding Techniques of Chinese Materia Medica, Chongqing 400065, China;3. Chongqing Subcenter of National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Science, Chongqing 400065, China
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Abstract:

In order to screen fungi with promoting effect on Artemisia annua growth, the endophytic fungi were isolated from A. annua brunches by tissue isolation method and molecular identified. The promoting effect of Trichoderma atroviride was studied. The results showed that 23 endophytic fungi were isolated belonging to 16 speices, in which the genus Colletotrichum was dominant. The fermentation broth of T. atroviride had significantly negative effect on seed germination and seedling growth of A. annua The mycelium of T. atroviride liquid fermentation had significant promoting effect on seedling growth of A. annua during April to May, but did not after July. So, it was suggested that T. atroviride could be used as fertilizer for seedlings of A. annua, and it combining with chemical fertilizer could also be applied to adult plants.

Key words:Artemisia annua;Endophytic fungi;Trichoderma atroviride;Promoting effect

Reference

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伍晓丽,崔广林,刘飞,李隆云.青蒿内生真菌分离、分子鉴定及深绿木霉对青蒿的促生作用研究[J].热带亚热带植物学报,2018,26(1):56~64

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Received:May 16,2017
Revised:August 01,2017
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Online: January 30,2018
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