Home > Archive>Volume 25, Issue 6, 2017 >587-593. DOI:10.11926/jtsb.3766
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Studies on H2O2Induced Effect on Huperzia serrata in vitro
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    Abstract:

    In order to gain high yield strain of huperzine A (HupA) from Huperzia serrata (Thunb.) Trev. in vitro, the hydrogen peroxide (H2O2) induced thallus was studied. The results showed that the HupA content in induced thallus significantly increased. The strain SH42 had highest yield of HupA with relative growth rate and HupA content for 4499.28% and 261.17 μg g-1 DW, respectively, which increased 2.35 times and 2.43 times of the initial strain, respectively. Moreover, there were significant difference spectral band in SOD isozymes and soluble protein among strains. The variation characteristics were stable after nine generations. Therefore, it was suggested that H2O2 had good induced effect on thallus of Huperzia serrata, which could be used for screening high-yield strain of HupA in vitro.

    Reference
    [1] LIU J S, YU C M, ZHOU Y Z, et al. Study on the chemistry of huperzine A and B[J]. Acta Chim Sin, 1986, 44(10):1035-1040. 刘嘉森, 俞超美, 周有作, 等. 石杉碱甲和石杉碱乙的化学研究[J]. 化学学报, 1986, 44(10):1035-1040.
    [2] ZHANG H Y, TANG X C. Neuroprotective effects of huperzine A:New therapeutic targets for neurodegenerative disease[J]. Trends Pharmacol Sci, 2006, 27(12):619-625. doi:10.1016/j.tips.2006.10. 004.
    [3] RATIA M, GIMÉNEZ-LLORT L, CAMPS P, et al. Huprine X and huperzine A improve cognition and regulate some neurochemical processes related with alzheimer's disease in triple transgenic mice (3xTg-AD)[J]. Neurodegener Dis, 2013, 11(3):129-140. doi:10.1159/000336427.
    [4] QIAN Z M, KE Y. Huperzine A:Is it an effective disease-modifying drug for Alzheimer's disease?[J]. Front Aging Neurosci, 2014, 6:216. doi:10.3389/fnagi.2014.00216.
    [5] MA X Q, TAN C H, ZHU D Y, et al. Huperzine A from Huperzia species:An ethnopharmacolgical review[J]. J Ethnopharmacol, 2007, 113(1):15-34. doi:10.1016/j.jep.2007.05.030.
    [6] LIANG H, PAN L H, LUO J P. Research progress on sustainable utilization of huperzine A resources[J]. J Anhui Agric Sci, 2010, 38(23):12467-12468. doi:10.13989/j.cnki.0517-6611.2010.23.261. 梁昊, 潘利华, 罗建平. 石杉碱甲资源可持续利用研究进展[J]. 安徽农业科学, 2010, 38(23):12467-12468. doi:10.13989/j.cnki.0517-6611.2010.23.261.
    [7] LIU J H, MA Y Z, ZHANG J L, et al. Study on lateral buds induction and culture of Huperzia serrata[J]. Chin Trad Herb Drugs, 2016, 47(17):3103-3108. doi:10.7501/j.issn.0253-2670.2016.17.024. 刘江海, 马英姿, 张家玲, 等. 蛇足石杉侧芽的发生与培养研究[J]. 中草药, 2016, 47(17):3103-3108. doi:10.7501/j.issn.0253-2670.2016. 17.024.
    [8] LI X J, YANG X F, ZHU W N, et al. Plant regeneration of Huperzia serrata by rapid propagation of shoot tips[J]. Mod Chin Med, 2014, 16(5):387-390. doi:10.13313/j.issn.1673-4890.2014.05.010. 李晓君, 杨雪飞, 朱皖南, 等. 蛇足石杉茎尖组织培养研究[J]. 中国现代中药, 2014, 16(5):387-390. doi:10.13313/j.issn.1673-4890. 2014.05.010.
    [9] JI Z D, TU Y S, DING M H, et al. In vitro culture of Huperzia serrata thallus for the medicinal component production[J]. Nat Prod Res Dev, 2014, 26(5):645-649,720. doi:10.16333/j.1001-6880.2014.05.004. 吉枝单, 涂艺声, 丁明华, 等. 蛇足石杉离体培养产生有效成分的研究[J]. 天然产物研究与开发, 2014, 26(5):645-649,720. doi:10. 16333/j.1001-6880.2014.05.004.
    [10] JI Z D, TU Y S, CHEN M, et al. Conditional optimization and kinetic research on producing huperzine A using Huperzia serrata in vitro[J]. Chin Trad Herb Drugs, 2016, 47(3):488-492. doi:10.7501/j.issn. 0253-2670.2016.03.023. 吉枝单, 涂艺声, 陈曼, 等. 蛇足石杉离体培养产石杉碱甲的条件优化及动力学研究[J]. 中草药, 2016, 47(3):488-492. doi:10.7501/j.issn.0253-2670.2016.03.023.
    [11] Xu X Z, Tu Y S, JI Z D, et al. In vitro-cultured morphological changes in Huperzia serrata and accumulation of huperzine A[J]. Chin Bull Bot, 2015, 50(6):733-738. doi:10.11983/CBB14220. 徐贤柱, 涂艺声, 吉枝单, 等. 蛇足石杉离体培养物形态变化与石杉碱甲积累的研究[J]. 植物学报, 2015, 50(6):733-738. doi:10. 11983/CBB14220.
    [12] TERMINI J. Hydroperoxide-induced DNA damage and mutations[J]. Mutat Res, 2000, 450(1/2):107-124. doi:10.1016/S0027-5107(00)00019-1.
    [13] CHEN M, TU Y S, YE L N, et al. Effect of amino acids on thallus growth and huperzine A accumulation in Huperzia serrata[J]. Chin Bull Bot, 2017, 52(2):218-224. doi:10.11983/CBB16033. 陈曼, 涂艺声, 叶丽婻, 等. 氨基酸对蛇足石杉叶状体增殖及石杉碱甲积累的影响[J]. 植物学报, 2017, 52(2):218-224. doi:10. 11983/CBB16033.
    [14] DONG L H, FAN S W, LING Q Z, et al. Study on huperzine A-producing endophytic fungi isolated from Huperzia serrata[J]. Nat Prod Res Dev, 2014, , 26(5): 650–655. doi: 10.16333/j.1001-6880.2014. 05.001. 范三微, 凌庆枝, 等. 千层塔中产石杉碱甲内生真菌的研究[J]. 天然产物研究与开发, 2014, 26(5):650-655. doi:10.16333/j. 1001-6880.2014.05.001.
    [15] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem, 1976, 72(1/2):248-254.
    [16] LUO G H, WANG A G. Studies on gel electrophoresis and activity of SOD in plants[J]. Plant Physiol Commun, 1983, (6):44-45. doi:10. 13592/j.cnki.ppj.1983.06.024. 罗广华, 王爱国. 植物SOD的凝胶电泳及活性的显示[J]. 植物生理学报, 1983(6):44-45. doi:10.13592/jcnki.ppj.1983.06.024.
    [17] GU R S, LIU Q L, CHEN X M, et al. Comparison and optimization of the methods on protein extraction and SDS-PAGE in woody plants[J]. Chin Bull Bot, 1999, 16(2):171-177. doi:10.3969/j.issn.1674-3466. 1999.02.011. 谷瑞升, 刘群录, 陈雪梅, 等. 木本植物蛋白提取和SDS-PAGE分析方法的比较和优化[J]. 植物学通报, 1999, 16(2):171-177. doi:10.3969/j.issn.1674-3466.1999.02.011.
    [18] TU Y S, WU X C. Clone screening of high yield of flavonoids from grass coral[J]. Chin Trad Herb Drugs, 2006, 37(2):276-279. doi:10. 3321/j.issn:0253-2670.2006.02.044. 涂艺声, 吴笑臣. 筛选草珊瑚黄酮高产细胞克隆系的研究[J]. 中草药, 2006, 37(2):276-279. doi:10.3321/j.issn:0253-2670.2006.02. 044.
    [19] KIM M, AHN J, JEON H, et al. Development of a Dunaliella tertiolecta strain with increased zeaxanthin content using random mutagenesis[J]. Mar Drugs, 2017, 15(6):189. doi:10.3390/md15060189.
    [20] LI X Y, SUN Y X, SONG L P, et al. Effect of UV-B radiation on POD and SOD isoenzyme of Arabidopsis thaliana[J]. SW Chin J Agric Sci, 2011, 24(6):2143-2146. doi:10.3969/j.issn.1001-4829.2011.06.024. 李晓阳, 孙永星, 宋丽平, 等. UV-B辐射对拟南芥POD、SOD同工酶的影响[J]. 西南农业学报, 2011, 24(6):2143-2146. doi:10.3969/j.issn.1001-4829.2011.06.024.
    [21] GAO W Y, LI K F, GAO X M, et al. Effects of space environment on DNA mutagenesis and secondary metabolites in Glycyrrhiza uralensis[J]. Chin Sci Bull, 2008, 38(11):1090-1094. doi:10.3724/SP.J.1005. 2008.01083. 高文远, 李克峰, 高秀梅, 等. 太空环境对甘草DNA诱变作用和次生代谢产物的影响[J]. 中国科学C辑:生命科学, 2008, 38(11): 1090–1094. doi: 10.3724/SP.J.1005.2008.01083.
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叶丽婻,涂艺声,黄倩,余晓,袁慧慧,鲁淑敏. H2O2对蛇足石杉离体培养物诱变效应的研究[J].热带亚热带植物学报,2017,25(6):587~593

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History
  • Received:May 10,2017
  • Revised:July 13,2017
  • Online: December 04,2017
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