Cloning and Expression Analysis of miR398 and miR408 in Phyllostachys edulis

Abstract:

To investigate the expression patterns of miR398 and miR408 in Phyllostachys edulis, the precursor sequences of ped-MIR398 and ped-MIR408 were isolated, and the expression of miRNAs was analyzed by real-time quantitative PCR (qPCR). The results showed that the length of precursor sequences of ped-MIR398 and ped-MIR408 were 83 bp and 92 bp, respectively, which both could fold into stable stem-loop structures, and the mature sequences (ped-miR398 and ped-miR408) were generated at 5' end of the arm in the stem-loop structures, respectively. The qPCR results indicated that both ped-miR398 and ped-miR408 were constitutively expressed, among which was most abundant in leaf blades. The expression of ped-miR398 and ped-miR408 were both up-regulated by the treatments of high light intensity, sucrose and GA₃, and they were both down-regulated by the treatments of CuSO₄ and ABA. Under the treatments of darkness, NaCl and 4℃, the expression of ped-miR398 was up-regulated, and that of ped-miR408 was down-regulated. Therefore, it was suggested that ped-miR398 and ped-miR408 might play different regulatory roles in the process of abiotic stress adaptation in bamboo.

Key words:Phyllostachys edulis;miRNA;Abiotic stress;Gene expression