Home > Archive>Volume 25, Issue 4, 2017 >348-356. DOI:10.11926/jtsb.3688
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Effects of Exogenous Ascorbic Acid and Methy Jasmonate on Ethephon-induced Leaf Senescence in Rice
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School of Life Science,South China Normal University,School of Life Science,South China Normal University,Guangdong Key Laboratory of Biotechnology for Plant Development,School of Life Science,South China Normal University;China

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    Abstract:

    The effects of exogenous ascorbic acid (AsA) and methy jasmonate (MeJA) on rice (Oryza sativa) leaf senescence induced by ethephon was investigated using wild type of cultivar line Zhonghua 11(ZH-11) and the mutant line GI-2 with down-regulated GLDH gene,which GLDH was a key enzyme in AsA biosynthesis path.The results showed that exogenous AsA increased ascorbic acid content in GI-2.Likewise,the contents of Rubisco protein and chlorophyll contents increased significantly after treated by AsA,and the descending rate of photosynthetic parameters decreased,while AsA treatment had no significant effects on ZH-11.In both cultivars,MeJA significantly decreased the contents of ascorbic acid,Rubisco protein and chlorophyll after treatment,while increased the descending rate of photosynthetic parameters,and had a stronger effect on ZH-11 than GI-2.Therefore,it was demonstrated that exogenous AsA plays an effective role in alleviating the early-senescence of rice leaf induced by ethephon,while MeJA has the opposite effects.

    Reference
    [1] ZHANG W X, PENG C R, HU S X, et al. Advances of research on early senescence of rice leaf and phytohormones regulation[J]. Acta Agri Jiangxi, 2007, 19(5):27-31. doi:10.3969/j.issn.1001-8581.2007. 05.009. 张文学, 彭春瑞, 胡水秀, 等. 水稻叶片早衰及其外源激素的调控研究进展[J]. 江西农业学报, 2007, 19(5):27-31. doi:10.3969/j.issn. 1001-8581.2007.05.009.
    [2] LU D Z, PAN Y C, MA Y F, et al. Physiological and biochemical studies on leaf senescence at heading and grain formation stage in hybrid rice[J]. Sci Agri Sin, 1988, 21(3):21-26. 陆定志, 潘裕才, 马跃芳, 等. 杂交水稻抽穗结实期间叶片衰老的生理生化研究[J]. 中国农业科学, 1988, 21(3):21-26.
    [3] XU Z H, LI J Y. Plant hormones research in China:Past, present and future[J]. Chin Bull Bot, 2006, 23(5):433-442. 许智宏, 李家洋. 中国植物激素研究:过去、现在和未来[J]. 植物学通报, 2006, 23(5):433-442.
    [4] GUO W Q, CHEN B L, LIU R X, et al. Effects of nitrogen application rate on cotton leaf antioxidant enzyme activities and endogenous hormone contents under short-term waterlogging at flowering and boll-forming stage[J]. Chin J Appl Ecol, 2010, 21(1):53-60. 郭文琦, 陈兵林, 刘瑞显, 等. 施氮量对花铃期短期渍水棉花叶片抗氧化酶活性和内源激素含量的影响[J]. 应用生态学报, 2010, 21(1):53-60.
    [5] JING H C, SCHIPPERS J H M, HILLE J, et al. Ethylene-induced leaf senescence depends on age-related changes and OLD genes in Arabidopsis[J]. J Exp Bot, 2005, 56(421):2915-2923. doi:10.1093/jxb/eri287.
    [6] HONG Z, ZHANG Y Z, CHEN H B, et al. The physiological function, biological and chemical synthesis of natural physiological active substance of jasmonates and its methyl ester[J]. Pesticides, 2000, 39(5):8-11. doi:10.3969/j.issn.1006-0413.2000.05.003. 洪忠, 张永忠, 陈虎保, 等. 天然生理活性物质茉莉酸及其甲酯的生理作用与生物、化学合成[J]. 农药, 2000, 39(5):8-11. doi:10. 3969/j.issn.1006-0413.2000.05.003.
    [7] WU J S, ZHONG K. The molecular biology research on the action of jasmonates[J]. Chin Bull Bot, 2002, 19(2):164-170. doi:10.3969/j. issn.1674-3466.2002.02.005. 吴劲松, 种康. 茉莉酸作用的分子生物学研究[J]. 植物学报, 2002, 19(2):164-170. doi:10.3969/j.issn.1674-3466.2002.02.005.
    [8] SMIMOFF N. BOTANICAL BRIEFING:The function and metabolism of ascorbic acid in plants[J]. Ann Bot, 1996, 78(6):661-669. doi:10.1006/anbo.1996.0175.
    [9] ZHENG Q W, WANG X K, XIE J Q, et al. Effects of exogenous ascorbate acid on membrane protective system of in situ rice leaves under O3 stress[J]. Acta Ecol Sin, 2006, 26(4):1131-1137. doi:10. 3321/j.issn:1000-0933.2006.04.021. 郑启伟, 王效科, 谢居清, 等. 外源抗坏血酸对臭氧胁迫下水稻叶片膜保护系统的影响[J]. 生态学报, 2006, 26(4):1131-113. doi:10.3321/j.issn:1000-0933.2006.04.021.
    [10] WHEELER G L, JONES M A, SMIRNOFF N. The biosynthetic pathway of vitamin C in higher plants[J]. Nature, 1998, 393(6683):365-369. doi:10.1038/30728.
    [11] CONKLIN P L. Recent advances in the role and biosynthesis of ascorbic acid in plants[J]. Plant Cell Environ, 2001, 24(4):383-394. doi:10.1046/j.1365-3040.2001.00686.x.
    [12] YU L, LIU Y H, PENG X X. Cloning, prokaryotic expression of rice L-galactono-1,4-lactone dehydrogenase gene and preparation of anti-GLDH antibodies[J]. J Hunan Agri Univ (Nat Sci), 2010, 36(4):381-384,429. doi:10.3724/SP.J.1238.2010.00381. 俞乐, 刘拥海, 彭新湘. 水稻L-半乳糖内酯脱氢酶基因的克隆和原核表达及抗体的制备[J]. 湖南农业大学学报(自然科学版), 2010, 36(4):381-384,429. doi:10.3724/SP.J.1238.2010.00381.
    [13] TAMAOKI M, MUKAI F, ASAI N, et al. Light-controlled expression of a gene encoding L-galactono-γ-lactone dehydrogenase which affects ascorbate pool size in Arabidopsis thaliana[J]. Plant Sci, 2003, 164(6):1111-1117. doi:10.1016/S0168-9452(03)00122-5.
    [14] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△CT method[J]. Methods, 2001, 25(4):402-408. doi:10.1006/meth.2001.1262.
    [15] GILLESPIE K M, AINSWORTH E A. Measurement of reduced, oxidized and total ascorbate content in plants[J]. Nat Protoc, 2007, 2(4):871-874. doi:10.1038/nprot.2007.101.
    [16] AMON D I. Copper enzymes in isolated chloroplasts:Polypheno-loxidase in Beta vulgaris[J]. Plant Physiol, 1949, 24(1):1-15. doi:10. 1104/pp.24.1.1.
    [17] TU Z P, CAI W J, LIN X Z, et al. Photoinhibition and rice productivity[J]. Jiangsu J Agri Sci, 1990, 6(S):1-15. 屠曾平, 蔡惟涓, 林秀珍, 等. 光抑制与水稻生产力[J]. 江苏农业学报, 1990, 6(S):1-15.
    [18] WEAVER L M, GAN S S, QUIRIN B, et al. A comparison of the expression patterns of several senescence-associated genes in response to stress and hormone treatment[J]. Plant Mol Biol, 1998, 37(3):455-469. doi:10.1023/A:1005934428906.
    [19] ZHU C, ZENG G W. Effect of 4PU-30 on leaf senescence and active oxygen metabolism in rice[J]. J Zhejiang Univ (Agri Life Sci), 2000, 26(5):483-488. doi:10.3321/j.issn:1008-9209.2000.05.006. 朱诚, 曾广文. 4PU-30对水稻叶片衰老与活性氧代谢的影响[J]. 浙江大学学报(农业与生命科学版), 2000, 26(5):483-488. doi:10. 3321/j.issn:1008-9209.2000.05.006.
    [20] GUAN J F, MA Z H, LI M X, et al. Inhibitory effects of spermidine on pollen germination and pollen tube elongation of apple and both relation to Ca2+[J]. Plant Physiol Commun, 2000, 36(2):107-110. 关军锋, 马智宏, 李敏霞, 等. 亚精胺对苹果花粉萌发与花粉管伸长的抑制效应及其与Ca2+的关系[J]. 植物生理学通讯, 2000, 36(2):107-110.
    [21] ASADA K. The water-water cycle as alternative photon and electron sinks[J]. Philos Trans R Soc B Biol Sci, 2000, 355(1402):1419-1431. doi:10.1098/rstb.2000.0703.
    [22] NIE X Z, LIU D H, XU Z S. Role of ethylene in the senescence of detached rice leaves[J]. J Huazhong Agri Univ, 1989, 8(3):218-222. 聂先舟, 刘道宏, 徐竹生. 乙烯在离体水稻叶片衰老中的作用[J]. 华中农业大学学报, 1989, 8(3):218-222.
    [23] GUO W M, ZENG W Q, CHEN F D. Regulation of ethylene on senescence of cut chrysanthemum flower[J]. J Nanjing Agri Univ, 1997, 20(4):24-29. doi:10.7685/j.issn.1000-2030.1997.04.005. 郭维明, 曾武清, 陈发棣. 乙烯对切花菊衰老的调节[J]. 南京农业大学学报, 1997, 20(4):24-29. doi:10.7685/j.issn.1000-2030.1997. 04.005.
    [24] ZHOU X H, GU Z H, XU H N, et al. The effects of exogenous ascorbic acid on photosynthetic characteristics in Oryza sativa L. under aluminum stress[J]. J Yangzhou Univ (Agri Life Sci), 2015, 36(3):73-78. 周小华, 谷照虎, 徐慧妮, 等. 外源抗坏血酸AsA对铝胁迫下水稻光合特性的影响[J]. 扬州大学学报(农业与生命科学版), 2015, 36(3):73-78.
    [25] TANG M L. Effect of MJ on leaf senescence in vitro and mechanism[D]. Baoding:Hebei Agricultural University, 2002:17-20. 唐美玲. 茉莉酸甲酯对离体叶片衰老的影响及其作用机制[D]. 保定:河北农业大学, 2002:17-20.
    [26] JIN P. Study on regulation and mechanisms of postharvest disease and chilling injury of peach fruit after harvest[D]. Nanjing:Nanjing Agricultural University, 2009:44-47. 金鹏. 桃果实采后病害和冷害调控及其机理研究[D]. 南京:南京农业大学, 2009:44-47.
    [27] FARQUHAR G D, SHARKEY T D. Stomatal conductance and photo-synthesis[J]. Annu Rev Plant Physiol, 1982, 33(4):317-345. doi:10. 1146/annurev.pp.33.060182.001533.
    [28] XIE J Q, LI G X, WANG X K, et al. Effect of exogenous ascorbic acid on photosynthesis and growth of rice under O3 stress[J]. Chin J Eco-Agri, 2009, 17(6):1176-1181. doi:10.3724/SP.J.1011.2009. 01176. 谢居清, 李国学, 王效科, 等. 外源抗坏血酸对臭氧胁迫下水稻光合及生长参数的影响[J]. 中国生态农业学报, 2009, 17(6):1176-1181. doi:10.3724/SP.J.1011.2009.01176.
    [29] THOMAS H, STODDART J L. Leaf senescence[J]. Annu Rev Plant Physiol, 1980, 31:83-111. doi:10.1146/annurev.pp.31.060180.000503.
    [30] DESIMONE M, HENKE A, WAGNER E. Oxidative stress induces partial degradation of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase in isolated chloroplasts of barley[J]. Plant Physiol, 1996, 111(3):789-796. doi:10.1104/pp.111.3.789.
    [31] ISHIDA H, SHIMIZU S, MAKINO A, et al. Light-dependent fragmentation of the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase in chloroplasts isolated from wheat leaves[J]. Planta, 1998, 204(3):305-309. doi:10.1007/s004250050260.
    [32] LI R, ZHOU W, LI L, et al. Changes in relative content of Rubisco large subunit in naturally senescing rice leaves[J]. Chin J Rice Sci, 2009, 23(5):555-558. doi:10.3969/j.issn.1001-7216.2009.05.17. 李瑞, 周玮, 李丽, 等. 水稻叶片自然衰老过程中Rubisco大亚基的含量变化[J]. 中国水稻科学, 2009, 23(5):555-558. doi:10.3969/j. issn.1001-7216.2009.05.17.
    [33] WU W H, PAN R Z. Effect of jasmonic acid methyl ester on the photosynthesis of rice seedlings[J]. Acta Bot Sin, 1998, 40(3):256-262. 吴文华, 潘瑞炽. 茉莉酸甲酯对水稻幼苗光合作用的影响[J]. 植物学报, 1998, 40(3):256-262.
    [34] LIN N, LU H. Research progress in ascorbate peroxidase gene family in plants[J]. J Chengdu Univ (Nat Sci), 2011, 30(2):97-101. doi:10. 3969/j.issn.1004-5422.2011.02.001. 李娜, 陆海. 植物抗坏血酸过氧化物酶基因家族研究进展[J]. 成都大学学报(自然科学版), 2011, 30(2):97-101. doi:10.3969/j.issn. 1004-5422.2011.02.001.
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魏燕霞,陆俐娜,彭长连.外源施加AsA和MeJA对乙烯利诱导水稻叶片衰老的影响[J].热带亚热带植物学报,2017,25(4):348~356

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  • Received:October 27,2016
  • Revised:January 13,2017
  • Adopted:March 28,2017
  • Online: July 18,2017
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