Screening of Reference Genes in Ananas comosus var. bracteatus for qRT-PCR

Abstract:

In order to screen appropriate reference gene for real-time qPCR (qRT-PCR) in Ananas comosus var. bracteatus at different development stages, the expression stability of 10 housekeeping genes, such as EF1, UBQ, ACT, GADPH, Histone, TUA, TUB, 18S, elf-5a, and α-tubulin, were detected, and the expression of PetF in seedling of A. comosus var. bracteatus was studied. The results showed that the expression stability of these genes in albino and green seedlings was different at development stages. Histone and α-tubulin were optimum reference genes at different development stages. Compared between green and albino seedlings, the ideal reference gene combination were 18S, EF1 and α-tubulin. The expression of PetF gene had the same variation trends in green and albino seedlings at three development stages by using screening reference genes. So, it was confirmed that the reference genes screened were suitable for qRT-PCR of A. comosus var. bracteatus.

Key words:Ananas comosus var. bracteatus;Real-time qPCR;Reference gene