Abstract:In order to isolate and screen the taxane-producing endophytic fungi, there were 491 strains of endophytic fungi had been isolated and purified from young stems, barks and leaves of Taxus chinensis var. mairei. Twenty-five strains of endophytic fungi could produce taxane, in which four strains produce taxol, baccatin Ⅲ and 10-deacetyl baccatin, eight strains produce taxol and baccatin Ⅲ, one strain taxol and 10-deacetyl baccatin, one strain produce baccatin Ⅲ and 10-deacetyl baccatin, and six strains only produce taxol, and five strains only produce baccatin Ⅲ. Taxane-producing endophytic fungi derived from young stems, leaves and barks were eleven strains, nine strains and six strains, respectively. The yields of taxol, baccatin Ⅲ and 10-deacetyl baccatin Ⅲ were ranged from 0.64 to 9.87 μg L-1, 0.48 to 3.42 μg L-1, and 0.20 to 1.00 μg L-1, respectively. Therefore, the taxane-producing endophytic fungi had wide distribution and more strains in T. chinensis var. mairei, and it could be good materials for studying metabolic pathways of taxane diterpenoids, and also could provide more potential fungal resources for taxane anticancer drugs.
