Home > Archive>Volume 25, Issue 2, 2017 >157-162. DOI:10.11926/jtsb.3651
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Differences Analysis of Cellulase Activity and Its Related Gene Expression in Shoots of Camellia sinensis
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tea research institution anhui academy of agricultural sciences,tea research institution anhui academy of agricultural sciences,tea research institution anhui academy of agricultural sciences,tea research institution anhui academy of agricultural sciences,tea research institution anhui academy of agricultural sciences

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    Abstract:

    In order to understand the characteristics of cellulase in shoots of Camellia sinensis, the changes in cellulase activity and related gene expressions in three cultivars of C. sinensis were studied. The results showed that the cellulase activity in the three cultivars had significant difference. The activities of the endo-glucanase and exo-glucanase in ‘Pingyang Tezao’ and ‘Fuzao No. 2’ increased with leaf maturity, which was contrary to the ‘Shuchazao’. The β-glucosidase activity in the three cultivars was the lowest at stage of three leaves with a bud, and its activity in young parts was higher than that in maturity parts in shoots. Otherwise, the activity of β-glucosidase in tea shoots was higher than that of exo-glucanase. The expresions of five cellulase-related genes could be detected in shoots of three cultivars by semi-quantitative PCR, in which the expression of two homologous genes had significant difference. Therefore, the glucanase would play a role in quality formation of tea.

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周汉琛,杨停,雷攀登,丁勇,黄建琴.茶树新梢中纤维素酶活性及相关基因表达差异分析[J].热带亚热带植物学报,2017,25(2):157~162

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History
  • Received:July 22,2016
  • Revised:October 09,2016
  • Adopted:November 21,2016
  • Online: March 16,2017
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