Genetic Relationships among 19 Breeding Parents of Asparagus by ISSR

Abstract:

In order to understand the genetic relationship among of Asparagus officinalis L. germplasms, the genetic diversity of 19 asparagus parents were analyzed by ISSR. The results showed that 187 bands were amplified by 18 primers with polymorphism rate for 93.6%. UPGMA indicated the Nei's coefficient ranged from 0.56 to 0.80. These cultivars could be clustered into three groups according to average coefficient of 0.62. Asparagus officinalis ‘Green Dragon’ cultivar from US formed GroupⅠwhich had far relation with others. Group II consisted of some artificial breeding parents of ‘Gijnlim’ from Holland and ‘Grande’ from US. The other tested parents made up the Group III. The individuals derived from the same variety had large genetic distance. There were abundant genetic diversity among individuals of asparagus from ploidy breeding. Therefore, these would provide the theoretical basis and technical guidance for parent selection in hybrid breeding of asparagus.