Comparison of Several RNA Extracting Methods from Heartwood of Santalum album

Abstract:

In order to screen RNA extracting method from heartwood of Santalum album, five methods were compared, including Trizol method, improved CTAB method, SDS-phenol method, guanidine isothiocyanate-CTAB method, and guanidine isothiocyanate-SDS method. The results showed that it was difficult to extract total RNA by Trizol method and guanidine isothiocyanate-CTAB method, and the improved CTAB method, SDS-phenol method and guanidine isothiocyanate-SDS method could successfully extract total RNA. The SDS-phenol method was not suitable for RNA extraction, because A₂₆₀ nm/A₂₃₀ nm was less than 2.0 and total RNA yield was low with (27.94±1.06) μg g^-1. However, the 28S rRNA and 18S rRNA bands of total RNA extracted by improved CTAB method and guanidine isothiocyanate-SDS method were clear, A₂₆₀ nm/A₂₈₀ nm ranged from 1.8 to 2.0, A₂₆₀ nm/A₂₃₀ nm were more than 2.0, the yields were (79.06±4.22) and (107.00±1.36) μg g^-1, respectively. The Actin segments in total RNA extracted by improved CTAB method and guanidine isothiocyanate-SDS method could amplificated with the same size and sigle band. Therefore, the improved CTAB methodand and guanidine isothiocyanate-SDS method were effective methods to extract total RNA from heartwood of S. album.