QIU Jian
1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China;2. College of Agronomy, Hainan University, Haikou 570228, ChinaLIU Tong
1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China;2. College of Agronomy, Hainan University, Haikou 570228, ChinaWANG Fan
1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China;2. College of Agronomy, Hainan University, Haikou 570228, ChinaWEI Fang
1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China;2. College of Agronomy, Hainan University, Haikou 570228, ChinaGAO Hong-hua
1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China;2. College of Agronomy, Hainan University, Haikou 570228, ChinaYANG Wen-feng
1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China;2. College of Agronomy, Hainan University, Haikou 570228, ChinaWU Ming
1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China;2. College of Agronomy, Hainan University, Haikou 570228, ChinaLUO Shi-qiao
1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China;2. College of Agronomy, Hainan University, Haikou 570228, ChinaXIAO Xian-zhou
1. Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China;2. College of Agronomy, Hainan University, Haikou 570228, Chinainstitute of rubber research, Chinese Academy of Tropical Agricultural Sciences,institute of rubber research, Chinese Academy of Tropical Agricultural Sciences,institute of rubber research, Chinese Academy of Tropical Agricultural Sciences;gronomy of Hainan University,institute of rubber research, Chinese Academy of Tropical Agricultural Sciences,institute of rubber research, Chinese Academy of Tropical Agricultural Sciences,institute of rubber research, Chinese Academy of Tropical Agricultural Sciences,institute of rubber research, Chinese Academy of Tropical Agricultural Sciences,institute of rubber research, Chinese Academy of Tropical Agricultural Sciences,institute of rubber research, Chinese Academy of Tropical Agricultural Sciences
In order to reveal the function of MYB52 in wood development of Hevea brasiliensis, a G21 subgroup gene member of MYB transcription factor, named HbMYB52, was cloned from its transcriptom. The ORF of HbMYB5 was 2726 bp, encoding a protein with 242 amino acids. HbMYB5 had high expression in xylem. Overexpression of HbMYB52 in Arabidopsis thaliana had no significant difference in phenotype between transgenic and wild type plants, but it led to significant thickening of cell wall in interfascicular fiber, and inhibition of secondary wall formation in vessels and xylem fiber. The contents of lignin and cellulose decreased in transgenic lines 3 and 6, and the expressions of key enzyme genes involving the corresponding components biosynthesis were significantly descended in transgenic lines. However, the lignin deposition in transgenic line 8 was ectopic, and the expressions of genes related to lignin biosynthesis were active. Therefore, it was suggested that HbMYB52 might play dual roles in the regulation of secondary wall formation, which negatively regulated secondary wall formation of vascular and promoted cell wall thickening of interfascicular fibers in A. thaliana.
仇键,刘彤,王帆,魏芳,高宏华,杨文凤,吴明,罗世巧,校现周.巴西橡胶树HbMYB52基因的克隆及其在拟南芥中的表达[J].热带亚热带植物学报,2016,24(6):671~679
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