Abstract:In order to understand the difference between allopolyploid and its parents in expression level and alternative splicing pattern of SR30 gene when the allopolyploid had been synthesized, different tissues or organs were collected from allotetraploid plant Raphanobrassica and its maternal parent Raphanus sativus, paternal parent Brassica oleracea var. alboglabra. Full-length coding sequence (CDS) and 3' untranslated regions (3'UTR) were cloned by using RACE-PCR. The expression level based on qRT-PCR and relative expression of each mRNA isoforms by using semi-quantitative RT-PCR was detected. The results showed that there were 5 mRNA isoforms of RsSR30 and 4 mRNA isoforms of BoSR30 appeared in the allopolyploid. In addition, the expression of SR30 reflected an obvious diversity between different tissues or organs. The expression of SR30 reduced significantly in allopolyploid compared with its parental species. Based on the mRNAs, it was predicted that there are 3 proteins encoded by RsSR30 and 2 proteins encoded by BoSR30. The difference between each protein isoforms was reflected in the RS (rich in serine-arginine dipeptides) domains located in C-terminal. Therefore, both of expression and alternative splicing patterns of SR30 changed when the allopolyploid had been synthesized.
