Proteomics Differences Analysis of the Uppermost Internode between TGMS Rice Line Pei'ai 64S and Its eui Mutant Changxuan 3S

Abstract:

In order to understand the elongation mechanism of the uppermost internode of TGMS rice (Oryza sativa L.) at protein expression level, the proteins in the uppermost internodes of eui mutant ‘Changxuan 3S’ and ‘Pei’ai 64S’ (control) before 2 days to heading were isolated by two-dimensional (2-DE) electrophoresis. A 2-DE map with a high resolution and good reproducibility were obtained by Coomasie brilliant blue staining. Fifty differentially expressed protein spots from 2-DE map were analyzed using MALDI-TOF/TOF-MS peptide mass fingerprint method, in which thirty-one differential proteins were identified. Compared with Pe'iai 64S, there were eleven proteins up-regulated in Changxuan 3S, and twenty proteins down-regulated. All of thirty-one differential proteins were divided into 7 categories according to their function. It was suggested that these differential proteins might play roles in elongation of the uppermost internode in ‘Changxuan 3S’ at heading stage, and eui gene in rice might regulate the expression of these proteins in uppermost internode at heading stage, thus promote cell division, especially cell elongation.