Home > Archive>Volume 24, Issue 3, 2016 >273-279. DOI:10.11926/j.issn.1005-3395.2016.03.005
PDF HTML XML Export Cite reminder
Effects of Endogenous Ascorbic Acid on Seed Germination and Seedling Growth of Rice
Author:
Affiliation:

School of Life Sciences,School of Life Sciences,School of Life Sciences,School of Life Sciences,School of Life Sciences

  • Article
    • | |
  • Metrics
    • |
  • Reference [26]
    • |
  • Related [20]
    • |
  • Cited by [0]
    • | |
  • Comments
    Abstract:

    The aim was to understand the function of endogenous ascorbic acid in rice (Oryza sativa) seeds. The homozygote transgenic lines GO-2 with the up-regulated GLDH (the key enzyme that catalyzes the final step of Asc biosynthesis in plants gene) and GI-2 with the down-regulated GLDH had been established from the cultivar ‘Zhonghua 11’ (ZH-11). The effect of endogenous ascorbic acid on seed germination and seedling growth of rice was studied among ZH-11, GO-2 and GI-2. The results showed that the germination rate of GO-2 was 6% and 60% higher than that of wild type (ZH-11), and GI-2, respectively. After treatment with 1 mmol L-1 exogenous ascorbic acid, the germination rate of GI-2 increased by 22%, but no significant effect was observed in that of GO-2 and the wild type (ZH-11). The root length, plant height, tiller number and fresh weight of seedling in GO-2 were higher than those in ZH-11 or GI-2. The expression of GLDH gene in GO-2 leaves was higher than that in ZH-11 or GI-2, too. So, these indicate ascorbic acid plays an important role in maintaining seed vigor and improving germination and seedling growth of rice.

    Reference
    [1] MAPSON L W. Metabolism of ascorbic acid in plants: Part I. Function [J]. Annu Rev Plant Physiol, 1958, 9(1): 119-150. doi:10.1146/annurev.pp.09.060158.001003.
    [2] SHANG R G, WANG B Y. Effect of L-ascorbic acid on the germination of the old Maca (Lepidum meyenii Walp.) seeds [J]. Seed, 2013, 32(11): 38-41. doi:10.3969/j.issn.1001-4705.2013.11.011. 尚瑞广, 王兵益. 抗坏血酸对玛咖陈种子萌发的影响 [J]. 种子, 2013, 32(11): 38-41. doi:10.3969/j.issn.1001-4705.2013.11.011.
    [3] HUANG Z Y, OSBORNE D J. Endo-glycanhydrolases activities in Artemisia sphaerocephala (Asteraceae) mucilaginous achene germination process [J]. Acta Bot Sin, 2002, 44(6): 753-756.
    [4] LEON-KLOOSTERZIEL K M, van DE BUNT G A, ZEEVAART J A D, et al. Arabidopsis mutants with a reduced seed dormancy [J]. Plant Physiol, 1996, 110(1): 233-240. doi:10.1104/pp.110.1.233.
    [5] RAGHAVAN V. Induction of vivipary in Arabidopsis by silique culture: implications for seed dormancy and germination [J]. Amer J Bot, 2002, 89(5): 766-776. doi:10.3732/ajb.89.5.766.
    [6] FAN M H, ZHANG Y X, SHI G, et al. Effects of exogenous ascorbic acid on seed germination and growth of Brassica napus under seawater stress [J]. Chin J Oil Crop Sci, 2009, 31(1): 34-38. doi:10.3321/j.issn:1007-9084.2009.01.007. 范美华, 张义鑫, 石戈, 等. 外源抗坏血酸对油菜种子在海水胁迫下萌发生长的影响 [J]. 中国油料作物学报, 2009, 31(1): 34-38. doi:10.3321/j.issn:1007-9084.2009.01.007.
    [7] KE D S, SUN G C, WANG A G, et al. Relationship between ascorbic acid and germination of seeds [J]. Chin J Appl Environ Biol, 2003, 9(5): 497-500. doi:10.3321/j.issn:1006-687X.2003.05.012. 柯德森, 孙谷畴, 王爱国, 等. 抗坏血酸与种子萌发的关系 [J]. 应用与环境生物学报, 2003, 9(5): 497-500. doi:10.3321/j.issn:1006- 687X.2003.05.012.
    [8] YU L, LIU Y H, PENG X X. Cloning, prokaryotic expression of rice L-galactono-1,4-lactone dehydrogenase gene and preparation of anti-GLDH antibodies [J]. J Hunan Agri Univ (Nat Sci), 2010, 36(4): 381-384,429. doi:10.3724/SP.J.1238.2010.00381. 俞乐, 刘拥海, 彭新湘. 水稻L-半乳糖内酯脱氢酶基因的克隆和原核表达及抗体的制备 [J]. 湖南农业大学学报: 自然科学版, 2010, 36(4): 381-384,429. doi:10.3724/SP.J.1238.2010.00381.
    [9] GILLESPIE K M, AINSWORTH E A. Measurement of reduced, oxidized and total ascorbate content in plants [J]. Nat Protoc, 2007, 2(4): 871-874. doi:10.1038/nprot.2007.101.
    [10] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J]. Methods, 2001, 25(4): 402-408. doi:10.1006/meth.2001.1262.
    [11] WHEELER G L, JONES M A, SMIRNOFF N. The biosynthetic pathway of vitamin C in higher plants [J]. Nature, 1998, 393(6683): 365-369. doi:10.1038/30728.
    [12] CONKLIN P L. Recent advances in the role and biosynthesis of ascorbic acid in plants [J]. Plant Cell Environ, 2001, 24(4): 383-394. doi:10.1046/j.1365-3040.2001.00686.x.
    [13] YAN Q Z. Seed Science [M]. Beijing: China Agriculture Press, 2001: 91-118. 颜启传. 种子学 [M]. 北京: 中国农业出版社, 2001: 91-118.
    [14] SHEN Y H, XU Z J, LI W H, et al. Effects of vitamin C soaking on germination of alfalfa seeds under different salinity stress [J]. Seed, 2009, 28(7): 42-44. doi:10.3969/j.issn.1001-4705.2009.07.013. 申玉华, 徐振军, 李文辉, 等. 维生素C浸种对盐胁迫下紫花苜蓿种子发芽特性的影响 [J]. 种子, 2009, 28(7): 42-44. doi:10.3969/j. issn.1001-4705.2009.07.013.
    [15] YANG P, LUO Q X, ZHANG K K, et al. Effects of exogenous vitamin on germination of cucumber seed in salt stress [J]. J Changjiang Veget, 2010(7): 41-42. doi:10.3865/j.issn.1001-3547.2010.07.024. 杨萍, 罗庆熙, 张珂珂, 等. 外源维生素对盐胁迫下黄瓜种子萌发及幼苗生长的影响 [J]. 长江蔬菜, 2010(7): 41-42. doi:10.3865/j. issn.1001-3547.2010.07.024.
    [16] KHAN A, IQBAL I, SHAH A, et al. Alleviation of adverse effects of salt stress in Brassica (Brassica campestris) by pre-sowing seed treatment with ascorbic acid [J]. Amer-Euras J Agri Environ Sci, 2010, 7(5): 557-560.
    [17] JIANG X W, LI H Q, WANG J H. Physiological response of Scutellaria baicalensis seed germination and seedling to exogenous ascorbic acid under salt stress [J]. Plant Physiol J, 2015, 51(2): 166-170. doi:10.13592/j.cnki.ppj.2014.0501. 江绪文, 李贺勤, 王建华. 盐胁迫下黄芩种子萌发及幼苗对外源抗坏血酸的生理响应 [J]. 植物生理学报, 2015, 51(2): 166-170. doi:10.13592/j.cnki.ppj.2014.0501.
    [18] DONG X H, SONG M. Reasons of seed inferior change and its prevention and restoration [J]. China Seed Ind, 2002(1): 39-40. doi:10.3969/j.issn.1671-895X.2002.01.025. 董新红, 宋明. 种子劣变的原因及其防止与修复 [J]. 中国种业, 2002(1): 39-40. doi:10.3969/j.issn.1671-895X.2002.01.025.
    [19] GALLIE D R. Increasing vitamin C content in plant foods to improve their nutritional value: Successes and challenges [J]. Nutrients, 2013, 5(9): 3424-3446. doi:10.3390/nu5093424.
    [20] GALLIE D R. The role of L-ascorbic acid recycling in responding to environmental stress and in promoting plant growth [J]. J Exp Bot, 2013, 64(2): 433-443. doi:10.1093/jxb/ers330.
    [21] MÜLLER-MOULÉ P, CONKLIN P L, NIYOGI K K. Ascorbate deficiency can limit violaxanthin de-epoxidase activity in vivo [J]. Plant Physiol, 2002, 128(3): 970-977. doi:10.1104/pp.010924.
    [22] SMIRNOFF N. The function and metabolism of ascorbic acid in plant [J]. Ann Bot, 1996, 78(6): 661-669. doi:10.1006/anbo.1996.0175.
    [23] TOKUNAGA T, MIYAHARA K, TABATA K, et al. Generation and properties of ascorbic acid-overproducing transgenic tobacco cells expressing sense RNA for L-galactono-1,4-lactone dehydrogenase [J]. Planta, 2005, 220(6): 854-863. doi:10.1007/s00425-004-1406-3.
    [24] VELJOVIC-JOVANOVIC S D, PIGNOCCHI C, NOCTOR G, et al. Low ascorbic acid in the vtc-1 mutant of Arabidopsis is associated with decreased growth and intracellular redistribution of the antioxidant system [J]. Plant Physiol, 2001, 127(2): 426-435. doi:10.1104/pp. 010141.
    [25] TABATA K, OBA K, SUZUKI K, et al. Generation and properties of ascorbic acid-deficient transgenic tobacco cells expressing antisense RNA for L-galactono-1,4-lactone dehydrogenase [J]. Plant J, 2001, 27(2): 139-148. doi:10.1046/j.1365-313x.2001.01074.x.
    [26] LIU Y H, YU L, WANG R Z. Level of ascorbic acid in transgenic rice for L-galactono-1,4-lactone dehydrogenase overexpressing or suppressed is associated with plant growth and seed set [J]. Acta Physiol Plant, 2011, 33(4): 1353-1363. doi:10.1007/s11738-010- 0669-5.
    Comments
    Comments
    分享到微博
    Submit
Get Citation

张启雷,刘强,高辉,陆俐娜,彭长连.内源抗坏血酸对水稻种子萌发及幼苗生长的影响[J].热带亚热带植物学报,2016,24(3):273~279

Copy
Share
Article Metrics
  • Abstract:1799
  • PDF: 2211
  • HTML: 0
  • Cited by: 0
History
  • Received:November 10,2015
  • Revised:December 16,2015
  • Adopted:March 01,2016
  • Online: May 19,2016
Article QR Code
You are the first4650358 Visitors
Governing Body：中国科学院
Organizer：中国科学院华南植物园 广东省植物学会
Address：Xingke Road 723,Tianhe District,Guangzhou,China
Phone：+86 (0)20-3725 2514
Journal of Tropical and Subtropical Botany ® 2025 All Rights Reserved