Abstract:The mung bean (Vigna radiata) seedlings in various states, including normal growth, inactivation and different levels of osmotic stress, were cultured under the same conditions, and the level of hydroxyl radicals in the culture environment and the respiration rate of seedlings were continuously measured. The results showed that the enviromental hydroxy radial surrounding normal growth seedlings was significantly higher than that of the environment without plant growth, however, under the same conditions, the inactivated seedlings had no significant effect on the level of surrounding enviromental hydroxyl radicals. Under osmotic stress, the effect of mung bean seedling on the level of hydroxyl radical in the atmosphere was very significant, and the influences on the levels of atmosphere hydroxyl radicals were different with different degree of osmotic stress. The relationship was close between the effect of mung bean seedling on the level of environmental hydroxyl radical and its respiration rate. Therefore, it was demonstrated that the level of hydroxyl radical was affected by the growth of mung bean seedling, and as well as, the effect was depend on the metabolic processes and the growth state of seedlings.