Expression Characteristics and Promoter Activity Analysis of PeSCL3 Gene from Phyllostachys edulis

Abstract:

In order to reveal the expression characteristics and promoter activity of SCL3 in Phyllostachys edulis, the open reading frame (ORF) of PeSCL3 and its upstream promoter sequence (PeSCL3p) were cloned from P. edulis by using homologous cloning method. Sequence analysis showed that the length of PeSCL3 ORF was 1335 bp, encoding a protein with 444 amino acids, which shared 93.9% homology with SCL3 from Oryza sativa. The length of PeSCL3p was 1358 bp, containing many kinds of cis-acting regulatory elements, such as abscisic acid response (ABRE), gibberellin-responsive element (GARE-motif and P-box), and drought-induced MYB binding sites, etc. Based on real-time quantitative PCR, the expression of PeSCL3 in leaf was the highest, following by stem and root, and the least in sheath. Besides, the expression of PeSCL3 was inhibited by GA₃, while it was induced by ABA, NaCl and drought. The GUS staining result of transgenic Arabidopsis thaliana with PeSCL3p::GUS showed that root tip, apical meristem and cotyledon petiole were all stained blue, especially in root tip with the deepest staining. So, these indicated that PeSCL3 might play an important role in regulating the growth and development of P. edulis, especially for that of root.