Home > Archive>Volume 24, Issue 2, 2016 >119-127. DOI:10.11926/j.issn.1005-3395.2016.02.001
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Application of BSP Method to the Methylation Detection of BZIP17 Homologous Genes in Allotetraploid Raphanobrassica and Its Parents
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College of Life Sciences, Wuhan University,College of Life Sciences, Wuhan University,College of Life Sciences, Wuhan University

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    Abstract:

    In order to understand the expression regulation mechanism of parent origin orthologous genes in the allopolyploid genome, the bisulfite sequencing PCR (BSP) method was optimized. The results showed that the methylaiton rate of BZIP17 homologous gene promoters in allotetraploid Raphanobrassica and its parents was ranged from 3.8% to 18.8%. Moreover, the expression level of BZIP17 genes in the allotetraploid and its parents were detected using quantitative real-time PCR, which summarized that the expression regulation of BZIP17 homologous genes is correlated with promoter methylation and other mechanisms. So, it was suggested that the BSP method could be applied to the methylation detection of more homologous genes, for the researches of molecular evolution patterns of allopolyploid homologous genes.

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黄笑君,吕昱树,王建波. BSP技术在萝卜-芥蓝异源四倍体及其亲本BZIP17同源基因甲基化检测中的应用[J].热带亚热带植物学报,2016,24(2):119~127

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History
  • Received:June 29,2015
  • Revised:September 09,2015
  • Adopted:October 26,2015
  • Online: March 28,2016
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