Establishment of the Regeneration System of Cotyledonary Node and Genetic Transformation of Pti4 Gene in Cowpea

Abstract:

In order to obtain genetic transformation system of cowpea (Vigna unguiculata), its cotyledon nodes with one cotyledon of ‘Chengjiang 7’ was used as explants, the regeneration system and Pti4 genetic transformation system by Agrobacterium-medium were studied. The results showed that the optimum mediums for aseptic seedling, adventitious bud introduction and adventitious bud elongation were MSB5 + 6-BA 3.0 mg L–1, MSB5 + 6-BA 1.0 mg L–1 + KT 0.06 mg L–1 and MSB5 + 6-BA 0.5 mg L–1 + IBA 0.2 mg L–1, respectively. Adventitious buds could rapidly rooting on MS medium to obtain full plantlets. The cotyledon node was used as receptor, the pit4 gene mediated by Agrobacterium was successfully transformed into the genome of resistant buds of ‘Chengjiang 7’. Therefore, the establishment of high efficient regeneration system for cowpea laid basis for studying on genetic transformation.