Qualitative and Quantitative Analysis of Diterpenoid Compounds in Sphagneticola trilobata (L.) Pruski

Abstract:

In order to understand the chemical constituents of Sphagneticola trilobata (L.) Pruski, eight diterpenoids were isolated from the ethanol extract of its whole plants. On the basis of spectral data, they were identified as ent-16β,17-dihydroxy-9(11)-kauren-19-oic acid (1), cussovantonin B (2), ent-16-kauren-19-oic acid (3), ent-3β-hydroxy-16-kauren-19-oic acid (4), 16α-hydroxy-ent-kauran-19-oic acid (5), 2β,16α-dihydroxy-ent-kauran-19-oic acid (6), 3α-angeloyloxypterokaurene L₃ (7) and pterokaurene L₃ (8). Compound 4 were isolated from genus Sphagneticola for the first time, and compounds 1, 2 and 6 were isolated from S. trilobata for the first time. Compound 3 showed strong antibacterial activity against several bacteria and further inhibited significantly α-glucosidase activity in vitro. Based on structural derivation with pentafluorobenzyl bromide coupled with GCMS techniques, the contents of compound 3 in the stem and leaves of S. trilobata were (1.00±0.21)mg g^-1 FW and (0.78±0.04) mg g^-1 FW, respectively.

Key words:Sphagneticola trilobata;Diterpenoids;Antibacterial;α-Glucosidase inhibitor;Quantitative analysis