Studies on Interaction between SoSGT1 and Proteins in Leaves of Chewing Cane Infected by Gibberella fujikuroi

Abstract:

In order to understand the interaction proteins with SoSGT1 during chewing cane (Saccharum officenarum L.) in denfense Gibberella fujikuroi, SoSgt1 and vector pGEX-6p-1 was used to construct vector pGEX-6p-1-sgt1, which expressed fusion protein of GST-SoSGT1. Seven proteins were obtained by GST pull down. The function of these proteins was classified into signal transduction, stress resistance and energy metabolism, in which might directly interact with SoSGT1, such as HSP90 and RAR1, and others might indirectly interact with SoSGT1. When the leaves of chewing cane ‘Fuan’ were infected by G. fujikuroi, the expression of coding genes of HSP70, 14-3-3 protein, 2-Cys-peroxiredoxin and pyridoxine biosynthesis protein was up-regulated, which indicated that these proteins might interact with SoSGT1 and associate with the resistance of chewing cane to G. fujikuroi.

Key words:Chewing cane;Gibberella fujikuroi;SoSGT1 protein;GST pull down;Protein interaction