Cloning and Bioinformatics Analysis of S-adenosylhomocysteine Hydrolase Gene form Kalanchoe daigremontiana

Abstract:

In order to understand the function of SAHH in Kalanchoe daigremontiana, a cDNA sequence, named as KdSAHH, was cloned by RT-PCR and RACE-PCR. The results showed that the full-length of KdSAHH cDNA was 1748 bp, encoding 485 amino acids. The predicted molecular weight (MW) of KdSAHH was about 53 kDa with estimated pI of 5.59-5.682. The Scanprostie and DNAstar prediction showed that KdSAHH protein with two conserved motifs was very conservative in evolution. There were high homology between KdSAHH and SAHHs in other species by amino acid alignment, and which had the closest relationship with that in Medicago sativa. Lupinus luteus used as template, there were differences in three-dimensional structure of KdSAHH simulated by SWISS-MODLE and Phyre. These would lay out basis for research in expression and function of KdSAHH.

Key words:Kalanchoe daigremontiana;S-adenosylhomocysteine hydrolase;SAHH;Clone;Bioinformatics