Screening of Reference Genes in Anthurium andraeanum Spathes for qRT-PCR Analysis

Abstract:

In order to screening stable reference genes that can be used to real-time quantitative PCR (qRT-PCR) analysis in Anthurium andraeanum spathes, five constitutively expressed genes, including EF1-a, UBQ7, ACTB, GADPH and His3, were analyzed expression stability by using qRT-PCR, and the expression of dihydroflavonol 4-reductase gene (dfr) was analyzed by using screened reference gene. The results showed that the expression stabilities of five reference genes were different among different cultivars. On the basis of the normalization factor V_n/n+1, the optimum number of reference gene was two. The expression of ACTB and UBQ7 were the most stable in dfr ferent cultivars and different development stages of A. andraeanum spathes, so that they were ideal reference genes. When ACTB and UBQ7 were used as reference genes, the gene dfr expressed in different cultivars and different development stages of A. andraeanum spathes, and the change trend of dfr expression were consistent. Thus, both ACTB and UBQ7 were suitable as reference genes for A. andraeanum spathes.