Abstract:In order to understand the chemical constituents of Rhodomyrtus tomentosa (Ait.) Hassk., ten compounds were isolated from alcohol extract of R. tomentosa leaves. On the basis of spectral data, they were identified as: lupeol (1), myricetin-3-O-α-L-rhamnoside (2), rhodomyrtone (3), 4,8,9,10-tetrahydroxy-2,3,7-trimethoxyanthracene- 6-O-β-D-glucopyranoside (4), stigmasterol (5), kaempferol-3-O-α-L-furanoarabinoside (6), myricetin (7), 23-hydroxytormentic acid (8), 2α,3β,19α,23-tetrahydroxyurs-12-en-28-oic acid β-D-glucopyranosyl ester (9) and laricitrin (10). Compounds 5-10 were obtained from this plant for the first time. Compound 3 showed antibacterial activity against Staphylococcus aureus, Bacillus cereus and B. subtilis with MIC=0.78 μg mL-1.