Screening of Proteins Interacting with Histone Deacetylase HDA705 in Rice (Oryza sativa) by Yeast Two Hybrid System

Abstract:

In order to understand the biological function of histone deacetylase HDA705 in rice, the yeast twohybrid bait vector of HDA705 and the cDNA library were constructed and the interaction partners of HDA705 were screened. The results showed that HDA705 bait vector showed no self-activating and no toxicity to yeast cells. Furthermore, the titer of cDNA library was suitable for normal yeast two-hybrid screening assay. There were 164 positive clones by yeast two-hybrid screening. DNA sequencing analysis indicated that these positive clones encoded 47 proteins which may interact with HDA705. These proteins included 3 transcription factors (or transcription co-factors) which play key roles in stress responses and hormone signaling pathways, 6 chloroplast located proteins which were involved in photosynthesis, 1 protein containing the R3H domain structure, and 22 enzymes in plant, etc. These may provide important cues to further study the biological function of HDA705 in rice.