Abstract:In order to understand the function of nucleotide-binding site disease resistance genes (NBS) in Musa acuminata, based on recent publication of draft genome sequence of M. acuminata, the genome-wide NBSencoding genes were identified, classified and chromosome located. The characteristics of gene duplications, phylogeny construction, distribution of adjacent promoter elements, expression evidences, and homologous function inferences were analyzed. The results showed that 125 NBS genes were identified from whole genome of M. acuminata, including 78 regular and 47 non-regular NBS genes. They were clustered into two clades phylogenetically, and most of the regular NBS-encoding genes resided in gene clusters and tandem duplications were predominant. Expression clues were found among 77 regular genes according to the public EST database. These would provide some fundamental information about the NBS-encoding genes in Musa species, and promote molecular disease-resistant breeding of cultivate banana.