Effect of Four Additives on Growth and Polysaccharide Content of Dendrobium officinale Protocorm-like Bodies

Abstract:

In order to understand the function of additive in medium of Dendrobium officinale, four additives, including coconut meat, sugarcane bagasse, banana peel and wheat bran, were supplied in 1/2MS medium, respectively. The effects of additives with different concentrates and culture time on growth and polysaccharide content of D. officinale PLBs were studied. The results showed that the PLBs proliferation, differentiation and the polysaccharide content were influenced by four additives. The proliferation rate of PBLs reached up to 146.1% supplemented with 15.0 g L^-1 sugarcane bagasse and cultured for 60 d. The polysaccharide content of PLBs was increased for 50.4% supplemented with 20.0 g L^-1 sugarcane bagasse and cultured for 40 days. So it was suggested that sugarcane bagasse was suitable additive for in vitro D. officinale PLBs owing to promote the growth of D. officinale PLBs and reduce cost of production.