Preliminary Study on Tissue Culture Technique in Brassica campestris L. ssp. chinensis var. utilis

Abstract:

In order to establish rapid propagation system of Brassica campestris ssp. chinensis var. utilis, the anthers as explants were in vitro cultured. The results showed that the anthers should be selected from unopened buds, which stigma was slightly higher than petal, and most of microspores were at uninucleate stage. The pollen germination rate was not high, and that in autumn and winter was higher than that in summer. The callus induction medium for anthers was MS+1.0 mg L^-1 KT+1.0 mg L^-1 2,4-D+3% sugar+6 g L^-1 agar+8% coconut milk (pH=5.8). The adventitious bud differentiation medium was MS+2.0 mg L^-1 6-BA+0.5 mg L^-1 NAA+1.0 g L^-1 active carbon+2% sugar+6 g L^-1 agar or MS+2.0 mg L^-1 ZT+0.5 mg L^-1 IAA+0.5 g L^-1 AgNO₃+1.0 g L^-1 active carbon+2% sugar+6 g L^-1 agar (pH=5.8). The adventitious bud rate inducted from anthers was 36.7%, and the regeneration plantlet rate was low owing to adventitious buds browning, while the regeneration plantlet rate reached to 80% induced from cotyledon or petioles.