Induction and Identification of Homologous Octoploid in Pogostemon cablin (Blanco) Benth.

Abstract:

In order to induce homologous octoploid of Pogostemon cablin, the effects of colchicine on induction rate were studied by using in vitro culture. Three kinds of explants, including adventitious buds, regenerated seedlings and calli, were used as material. The results showed that the induction rate of adventitious buds treated with 0.05% colchicine for 72 hours reached 85% accounting for total buds. The chromosome number of octoploid seedlings was 2n=8x=128, the roots and stems of octoploid P. cablin were thick and strong with large and dark leaves, small leaf index, and the stomata in lower epidermis were large and low density with much chloroplasts in guard cells, the mutant seedlings had good morphological traits. Therefore, the induction method would establish basis for breeding excellent strains of P. cablin with high yield and content of active ingredients.

Key words:Pogostemon cablin (Blanco) Benth.;Colchicine;Octoploid;Induction;Identification