Development and Application of SSR Molecular Markers from the Chloroplast Genome of Bamboo

Abstract:

In order to reveal the heterogeneity mechanism of ornamental bamboo leaves, the SSR molecular markers were developed from chloroplast genome of Dendrocalamus latiflorus and Bambusa oldhamii. The results showed that there were 87 and 86 SSR loci in chloroplast genome of D. latiflorus and B. oldhamii, respectively, in which the trinucleotide type was the most, followed by mononucleotide type. Based on the sequences of SSR loci in chloroplast genome, 21 pairs of SSR primers were designed. Six bamboo species could be amplified stable and clear bands with polymorphism by 11 pairs of primers, accounting for 52.4%. The cluster analysis indicated that 6 bamboo species could be divided into two groups, which was consisted with morphological classification. Meanwhile, polymorphism bands could be amplified in different parts of colorful leaves from Pleioblastus fortunei and Sasaella glabra f. albo-striata by 4 pairs of primers. So, it was suggested that these SSR markers could be used to distinguish the leaf heterogeneity of ornamental bamboo.

Key words:Bamboo;Chloroplast SSR;Heterogeneity