Proteomic Analysis of Loquat Peels under High Temperature and Strong Light Stresses

Abstract:

In order to understand the changes in protein components of loquat [Eriobotrya japonica (Thunb.) Lindl.] under high temperature and strong light stress, the responses of proteins in loquat ‘WDYDB' peels to stress were analyzed by using proteomic approaches, which fruits were sensitive to sunburn. The results showed that there were 31 protein dots with differential expression of more than two times on dimensional electrophoresis profiles, as compared between treatments of high temperature and strong light stresses and shading (Control). Among them, twenty-six differential proteins were identified by MALDI-TOF-TOF/MS analysis, including 11 down-regulated and 15 up-regulated proteins. These proteins could be divided into 4 categories according to their functions, including defense response, carbohydrate and energy metabolism, photosynthesis and other protein. The functions and roles of these proteins under high temperature and strong light stress were discussed. Therefore, it was suggested that these differential proteins could involve in responses to high temperature and strong light stresses.

Key words:Loquat peel;High temperature and strong light stresses;Sunburn;Differential expression protein