Cloning and Bioinformatics Analysis of ACT1 and ACT2 Genes from Eucalyptus camaldulensis

Abstract:

In order to reveal the biological function of Actin in growth and development of Eucalyptus camaldulensis, two fragments were cloned from the transcriptome database of E. camaldulensis seedlings at different developmental stages, named as EC-ACT1 and EC-ACT2, and their full-length cDNA were obtained by RACE method. Bioinformatics analysis showed that the full-length cDNA of EC-ACT1 and EC-ACT2 were 1533 bp and 1387 bp, respectively, and both with 1131 bp ORF encoding 377 amino acids. The amino acid sequence of Actin in E. camaldulensis had high similarity to those of other plants with typical conserved domains of Actin. Therefore, the EC-ACT1 and EC-ACT2 genes might play a role in regulating growth and development of E. camaldulensis.

Key words:Eucalyptus camaldulensis;Actin;Clone;Bioinformatics analysis