Construction of Fingerprinting and Genetic Diversity Analysis of Hemarthia Cultivars by EST-SSR

Abstract:

In order to understand the genetic diversity of Hemarthria cultivars, the DNA fingerprints of six Hemarthia cultivars were constructed by using 8 pairs of EST-SSR primers screened from 86 pairs, and the genetic diversities among them were analyzed. The results showed that eight pairs of ESR-SSR primers totally amplified 194 bands, in which 161 bands were polymorphic bands, accounting for 83.4%. The polymorphic information content (PIC) ranged from 0.480 to 0.695, with an average of 0.602. The UPGMA analysis showed that six cultivars of Hemarthia could be divided into two groups at the similarity coefficient of 0.652, and 6 cultivars could be completely separated by eight pairs of ESR-SSR primers. Based on electrophoretogram amplified by 3 pairs primers, the standard DNA fingerprinting for 6 Hemarthia cultivars were constructed, and each cultivars had an unique fingerprint. The average Nei's genetic diversity and Shannon's information index were 0.333 and 0.496, respectively. The similarity coefficient of 6 cultivars of Hemarthia ranged from 0.399 to 0.782. Therefore, it was suggested that 6 cultivars of Hemarthia had rich genetic diversity and significant differences between species.

Key words:EST-SSR;Hemarthria;Cultivar;DNA fingerprinting;Genetic diversity