Effect of in vitro Culture Conditions on Content of Plumbagin in Sundew (Drosera spatulata) Plantlets

Abstract:

In order to understand the effect of different culture conditions on content of plumbagin in sundew (Drosera spatulata) plantlets, the changes in plumbagin content were studied by using high performance liquid chromatography (HPLC). The results showed that the content of plumbagin in roots was higher than that in leaves. Light quality and organic element content in medium had no significant differences in plumbagin accumulation in sundew plantlets, but had significant effects on its growth. White light was the best for culture of sundew plantlets, followed by red light, blue light, and green light. Appropriate to reduce the organic matter content in medium could promote the growth and development of sundew plantlets. The effects of different plant growth regulator on plumbagin accumulation were in the order of 6-BA > NAA > KT > GA₃, and those on growth were in descending order: 6-BA > GA₃ > NAA > KT. Therefore, the optimum culture condition for sundew plantlets was 1/2MS as basic medium under white light, supplemented with 0 - 0.2 mg L^-1 6-BA, 0.2 mg L^-1 NAA, 0.5 mg L^-1 KT and 0.1 mg L^-1 GA₃.

Key words:Drosera spatulata;Plumbagin;HPLC;Light;Organic matter;Plant growth regulator